Alkaline Lysis Buffers A, B, C Recipes

Alkaline Lysis Buffer A Recip

       Add the following to 100ml distilled H2O

       5ml of 20% SDS

       2ml of 10M NaOH

Alkaline Lysis Buffer B Recipe

       Add the following to 100ml distilled H2O

       29.4g KAc

       11.5ml of Glacial Acetic Acid

Alkaline Lysis Buffer C Recipe

       Add the following to 900ml distilled H2O

       9g Glucose

       3g Tris

       20ml of 0.25M EDTA

       
q.s. to 1000ml with distilled H2O

       Autoclave using standard conditions

       Allow to cool to room temperature

       Store at 4℃

When ready to use add 0.02g lysozyme to 100ml of alkaline lysis buffer C to yield alkaline lysis buffer A.