Laboratory Protocols & SOPs Library

Bradford's Reagent Recipe

· Published 2023/07/27 · Updated 2025/06/09

Recipe

1.Dissolve 50mg of Coomassie Blue G250 in 50ml of methanol

2.Add 100ml of 85% H3PO4 to the solution from step 1

3.Add the solution from step 2 into 500ml of H2O and mix

4.Filter to remove and precipitates

5.Add an additional 350ml of H2O

6.Store at 4℃.

Caution: Always add acid slowly into water and do not add water into acid.

Categories: Laboratory Protocols & SOPs Library

Da — when not otherwise indicated, molecular weight units are daltons. Mw — weight-average molecular weight. Mn — number-average molecular weight.

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.

Cite this article

Aladdin Scientific. "Bradford's Reagent Recipe" Aladdin Knowledge Base, updated 2025/06/09. https://www.aladdinsci.com/us_ja/faqs/bradfords-reagent-recipe-en.html

Was this article helpful? Yes No 7 out 14 found this helpful