Dissolve 37 grams per liter of distilled/deionized (DDI) water. Heat with stirring. Boil 1 minute until completely solubilized. Adjust pH as necessary. Dispense into appropriate containers. Loosen caps or cover with foil. Autoclave for 15 minutes at 121ºC (15psi). Aseptically add 5% lysed horse blood and 10ml of filter sterilized aqueous solution of antimicrobials.
Component
g/liter
Enzymatic Digest of Animal Tissue
10g
Enzymatic Digest of Casein
10g
Sodium chloride
5g
Agar
12g
Total: 37g/Liter
Storage and Stability:
Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.
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