Recombinant Human CLPTM1 Protein: Usage & Handling Guide

Recombinant Human CLPTM1 Protein is a research-grade reagent designed for scientists investigating the role of Cleft Lip and Palate Transmembrane Protein 1 in apoptosis regulation, cell survival signaling, and cancer biology.

Product Name

Recombinant Human CLPTM1 Protein

SKU

rp369322

Supplier Reference

absea#PQ-554

Protein Origin

Human (Homo sapiens)

Application Area

Cell biology, apoptosis research, cancer research

Storage Recommendation

Store at -80°C upon receipt; avoid repeated freeze-thaw cycles

Handling

Reconstitute in appropriate buffer as indicated in the product datasheet; centrifuge briefly before use

Background: CLPTM1 and Its Biological Significance

CLPTM1 (Cleft Lip and Palate Transmembrane Protein 1) is a multi-pass transmembrane protein encoded by the CLPTM1 gene in humans. It was originally identified through positional cloning studies of cleft palate, and has since been found to be broadly expressed across multiple tissue types. Research has indicated that CLPTM1 plays roles in apoptosis regulation and cell survival pathways. Notably, overexpression of CLPTM1 has been observed in several cancers, implicating it as a potential oncology target and biomarker. Understanding CLPTM1 function at the molecular level requires well-characterized recombinant protein tools, making Recombinant Human CLPTM1 Protein an essential reagent for related investigations.

Reconstitution and Storage Protocol

Follow these steps to reconstitute and store Recombinant Human CLPTM1 Protein (rp369322) correctly:

1. Centrifugation: Upon receipt and before opening, briefly centrifuge the vial (e.g., 5–10 seconds at low speed) to collect any lyophilized material at the bottom of the tube.
2. Reconstitution: Reconstitute the protein using the buffer and volume specified in the product datasheet (typically sterile PBS or a carrier-protein-containing buffer). Add the reconstitution buffer gently along the side of the vial and allow the protein to dissolve at room temperature for 10 minutes without vortexing.
3. Aliquoting: Prepare single-use aliquots immediately after reconstitution to minimize freeze-thaw cycles.
4. Short-term storage: Store working aliquots at 4°C for up to 1 week.
5. Long-term storage: Store unused aliquots at -80°C. Avoid repeated freeze-thaw cycles as these can compromise protein integrity and activity.
6. Carrier protein: If the protein will be used at very low concentrations, consider adding a carrier protein such as BSA (0.1%) to improve stability.

Note: Always refer to the specific product datasheet (absea#PQ-554) for lot-specific reconstitution volumes, concentrations, and buffer formulations.

Experimental Applications and Usage Tips

Recombinant Human CLPTM1 Protein is suitable for a range of in vitro research applications. Typical uses include:

• Protein–protein interaction studies: Use as bait or prey in pull-down assays or co-immunoprecipitation experiments to identify CLPTM1 binding partners.
• Antibody validation: Serve as a positive control antigen for validating anti-CLPTM1 antibodies in Western blot, ELISA, or dot blot formats.
• Cell-based assays: Investigate CLPTM1's role in apoptosis and cell survival by supplementing cell culture models, following established protocols for recombinant protein delivery.
• Structural studies: Use purified protein as starting material for biophysical characterization (e.g., circular dichroism, dynamic light scattering) where applicable.

When setting up experiments, optimize protein concentration empirically and include appropriate negative controls. Pilot experiments at multiple concentrations (e.g., 0.1, 1, and 10 µg/mL) are recommended to establish dose-response relationships before committing to large-scale assays.

Quality, Safety, and Compliance Considerations

Quality: Recombinant Human CLPTM1 Protein from Aladdin Scientific is manufactured and quality-controlled to research-grade standards. Each lot is tested for purity and identity; refer to the Certificate of Analysis (CoA) provided with the product (supplier reference: absea#PQ-554) for lot-specific data including purity, endotoxin levels, and biological activity where applicable.

Safety: Although recombinant proteins derived from human sequences are generally considered low risk, standard laboratory biosafety practices should be followed. Wear appropriate personal protective equipment (PPE) including gloves, lab coat, and eye protection. Avoid inhalation or ingestion. Dispose of all protein-containing waste in accordance with institutional biosafety guidelines.

Compliance: This product is intended for research use only (RUO) and is not approved for diagnostic, therapeutic, or clinical use. Ensure that all experiments comply with your institution's ethical and biosafety regulations.

Troubleshooting Common Issues

If you encounter problems when working with Recombinant Human CLPTM1 Protein, consult the following troubleshooting guide:

• Protein will not dissolve after reconstitution: Allow more time (up to 30 minutes) at room temperature, or gently pipette up and down. Do not vortex. If aggregation is suspected, briefly centrifuge at low speed and collect the supernatant.
• Loss of activity after storage: Check that the protein was stored at -80°C and that freeze-thaw cycles were minimized. Confirm that the reconstitution buffer matches the datasheet recommendation. Consider adding a stabilizing carrier protein.
• Non-specific bands in Western blot: Optimize antibody concentrations and blocking conditions. Ensure the protein sample was not overloaded and that the loading buffer was compatible.
• High background in ELISA: Increase washing stringency and ensure all reagents were equilibrated to room temperature before use. Verify that coating concentration was appropriate for the assay format.

For further technical support, contact Aladdin Scientific's technical support team with your lot number and product SKU (rp369322).

Frequently asked questions

What is CLPTM1 and why is it relevant to cancer research?

CLPTM1 (Cleft Lip and Palate Transmembrane Protein 1) is a multi-pass transmembrane protein implicated in apoptosis regulation and cell survival signaling. Overexpression of CLPTM1 has been reported in various cancer types, making it a subject of interest as a potential oncology biomarker and therapeutic target. Recombinant Human CLPTM1 Protein enables researchers to study these functions in controlled in vitro settings.

How should I reconstitute Recombinant Human CLPTM1 Protein (rp369322)?

Briefly centrifuge the vial before opening to collect lyophilized material. Add the reconstitution buffer specified in the product datasheet (absea#PQ-554) gently along the vial wall, then allow the protein to dissolve at room temperature for approximately 10 minutes without vortexing. Aliquot immediately to minimize freeze-thaw cycles.

What are the recommended storage conditions for this protein?

Recombinant Human CLPTM1 Protein should be stored at -80°C for long-term preservation. Working aliquots may be kept at 4°C for up to one week. Repeated freeze-thaw cycles should be strictly avoided as they can degrade protein integrity and reduce biological activity.

Is Recombinant Human CLPTM1 Protein suitable for in vivo or clinical use?

No. This product is designated for research use only (RUO) and is not validated, approved, or intended for diagnostic, therapeutic, or clinical applications. All use must comply with institutional biosafety and ethical guidelines.

Who should I contact if I experience technical issues with rp369322?

Please contact Aladdin Scientific's technical support team. When reaching out, provide your product SKU (rp369322), the supplier reference number (absea#PQ-554), your lot number, and a detailed description of the issue encountered to enable efficient troubleshooting.

References

1. Product Datasheet: Recombinant Human CLPTM1 Protein, absea#PQ-554, Aladdin Scientific SKU rp369322.
2. UniProt entry for human CLPTM1 protein (Homo sapiens). UniProt Consortium.
3. General best practices for recombinant protein reconstitution and storage in research applications.

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.