Recombinant Human FAM177B Protein: FAQs & Key Facts

Recombinant Human FAM177B Protein is a research-grade recombinant protein derived from the human FAM177B gene, produced for use in life science studies requiring high-purity, functionally relevant protein material.

Product Name

Recombinant Human FAM177B Protein

SKU

rp376232

Species Origin

Human (Homo sapiens)

Protein Family

FAM177 family (Family with Sequence Similarity 177)

Supplier Reference

absea#PP-9837

Intended Use

In vitro research use only; not for diagnostic or therapeutic use

What Is Recombinant Human FAM177B Protein?

Recombinant Human FAM177B Protein (SKU: rp376232) is a recombinant form of the human FAM177B protein, a member of the FAM177 protein family characterized by conserved sequence similarity. The FAM177B gene encodes a protein whose precise biological function is still under active investigation in the research community. Recombinant versions of such proteins are valuable tools for exploring protein–protein interactions, antibody validation, structural studies, and functional assays in vitro.

This product is manufactured to research-grade standards and is intended exclusively for laboratory research applications. It is not approved or intended for use in clinical diagnostics, therapeutic procedures, or any in vivo applications in humans or animals.

Expression System, Purity, and Formulation

Recombinant proteins in the Aladdin Scientific catalog are typically produced using established heterologous expression systems such as Escherichia coli, insect cells (baculovirus), mammalian cells (HEK293 or CHO), or yeast, depending on the structural and post-translational modification requirements of the target protein. The specific expression host and purification method for rp376232 should be confirmed by consulting the official product datasheet or contacting Aladdin Scientific technical support, as these details are subject to lot-specific variation.

Purity is generally assessed by SDS-PAGE and, where applicable, by high-performance liquid chromatography (HPLC). Endotoxin levels are typically measured using the Limulus Amebocyte Lysate (LAL) assay. Specific purity and endotoxin values for rp376232 should be obtained from the Certificate of Analysis (CoA) supplied with each lot.

Storage, Stability, and Handling Recommendations

Proper storage and handling are critical to maintaining the activity and integrity of recombinant proteins. As a general guideline for research-grade recombinant proteins:

• Short-term storage: Store at 4 °C if the protein will be used within one to two weeks of receipt.
• Long-term storage: Aliquot the protein upon receipt to avoid repeated freeze–thaw cycles and store at −20 °C or −80 °C as recommended on the product datasheet.
• Carrier protein: If the protein is supplied in a carrier-free formulation at low concentration, consider adding a stabilizing agent such as BSA (0.1%) to prevent adsorption losses.
• Freeze–thaw cycles: Minimize the number of freeze–thaw cycles, as repeated cycling can lead to aggregation, loss of activity, and degradation.

Always refer to the specific storage conditions stated on the product datasheet and CoA for rp376232, as conditions may differ from general guidelines.

Recommended Applications and Experimental Use

Recombinant Human FAM177B Protein may be suitable for a range of in vitro research applications, subject to validation by the end user. Potential applications commonly associated with recombinant proteins of this type include:

• Antibody production and validation: Use as an immunogen or as a positive control antigen in Western blot, ELISA, or immunoprecipitation experiments.
• Binding and interaction studies: Investigate protein–protein or protein–ligand interactions using pull-down assays, surface plasmon resonance (SPR), or co-immunoprecipitation.
• Structural and biophysical studies: Assess secondary or tertiary structure using circular dichroism (CD) spectroscopy or other biophysical techniques.
• Functional assays: Where the biological activity of FAM177B is elucidated, the recombinant protein may be employed in cell-based or biochemical activity assays.

Users are advised to perform independent validation of the protein for each intended application and to refer to peer-reviewed literature for guidance on FAM177B biology.

Ordering, Quality, and Technical Support

Recombinant Human FAM177B Protein is available from Aladdin Scientific under SKU rp376232. To place an order, researchers and procurement specialists may visit the Aladdin Scientific website, contact a local distributor, or reach out to the Aladdin customer service team directly.

Each lot is accompanied by a Certificate of Analysis (CoA) confirming product identity, purity, and quality testing results. For technical questions regarding reconstitution protocols, buffer compatibility, activity confirmation, or application-specific guidance, please contact the Aladdin Scientific technical support team who can provide lot-specific documentation and expert advice.

For returns, refunds, or shipping inquiries, please consult the Aladdin Scientific customer service policies available on the company website or contact the support team directly.

Frequently asked questions

What is FAM177B and why is a recombinant form useful for research?

FAM177B (Family with Sequence Similarity 177 Member B) is a human protein encoded by the FAM177B gene. Its precise biological function remains under investigation. Recombinant Human FAM177B Protein provides researchers with a purified, well-defined protein source for studying its function, interactions, and potential role in cellular processes without relying on endogenous expression from cell or tissue lysates.

What expression system is used to produce rp376232, and what purity can I expect?

The specific expression system and purity specification for rp376232 (Recombinant Human FAM177B Protein) should be confirmed by reviewing the product datasheet and the Certificate of Analysis (CoA) provided with each lot. If you require this information prior to purchase, please contact Aladdin Scientific technical support for detailed lot-specific documentation.

How should I store Recombinant Human FAM177B Protein to maintain its activity?

In general, recombinant proteins should be aliquoted upon receipt to avoid repeated freeze–thaw cycles and stored at −20 °C or −80 °C for long-term stability, or at 4 °C if use is anticipated within one to two weeks. Always follow the specific storage instructions on the product datasheet and CoA for rp376232, as these take precedence over general guidelines.

Can Recombinant Human FAM177B Protein be used in cell-based functional assays?

The suitability of rp376232 for cell-based functional assays depends on the elucidation of FAM177B's biological activity and the assay design. Users should perform independent validation for each application. Aladdin Scientific technical support can advise on validated applications and provide guidance based on available data.

How do I place an order for rp376232 and what documentation is provided?

Recombinant Human FAM177B Protein (rp376232) can be ordered through the Aladdin Scientific website, through an authorized local distributor, or by contacting the Aladdin customer service team. Each shipment is accompanied by a Certificate of Analysis (CoA) confirming identity, purity, and lot-specific quality control data. Safety Data Sheets (SDS) are also available upon request.

Is Recombinant Human FAM177B Protein suitable for use as a Western blot positive control?

Recombinant Human FAM177B Protein can potentially serve as a positive control antigen in Western blot experiments where an anti-FAM177B antibody is being validated. However, users must confirm antibody compatibility and protein detectability under their specific denaturing or non-denaturing conditions. Independent validation is always recommended.

References

1. Aladdin Scientific product listing: Recombinant Human FAM177B Protein, SKU rp376232. Aladdin Scientific catalog.
2. Supplier reference: absea#PP-9837. Product characterization data on file with manufacturer.
3. General recombinant protein handling and storage best practices. Current Protocols in Protein Science. (General reference — specific chapter to be confirmed.)

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.