Recombinant Human GALNT9 Protein: FAQs & Key Facts

Recombinant Human GALNT9 Protein is a recombinantly expressed form of the human polypeptide N-acetylgalactosaminyltransferase 9 enzyme, supplied by Aladdin Scientific to support research into O-linked glycosylation pathways and related biological processes.

Product Name

Recombinant Human GALNT9 Protein

SKU

rp368036

Gene / Protein Family

GALNT9; polypeptide N-acetylgalactosaminyltransferase family (GalNAc-T)

Primary Function

Catalyzes the initial step of mucin-type O-linked glycosylation by transferring GalNAc from UDP-GalNAc to serine or threonine residues on target proteins

Supplier

Aladdin Scientific

Intended Use

Research use only (RUO)

What Is GALNT9 and Why Is It Important?

GALNT9 (Polypeptide N-Acetylgalactosaminyltransferase 9) is a member of the large GalNAc-transferase (GalNAc-T) enzyme family, which is responsible for initiating mucin-type O-linked protein glycosylation — one of the most abundant and diverse forms of post-translational modification in eukaryotes. These enzymes catalyze the transfer of N-acetylgalactosamine (GalNAc) from the sugar-nucleotide donor UDP-GalNAc to serine or threonine residues on acceptor proteins, forming the Tn antigen (GalNAc-α-O-Ser/Thr), which serves as the core 1 scaffold for elaboration of complex O-glycan structures.

Individual GalNAc-T family members differ in their tissue distribution, acceptor substrate specificity, and the sequence context they prefer to glycosylate. GALNT9 has been reported to exhibit a relatively restricted tissue expression pattern, making it of particular interest in studying cell-type-specific glycosylation events. Dysregulation of GalNAc-T enzymes, including GALNT9, has been linked to alterations in cell adhesion, signaling, and cancer biology, positioning this enzyme as a relevant target in disease research.

Product Specifications and Quality

Recombinant Human GALNT9 Protein (SKU: rp368036) is produced recombinantly to provide researchers with a consistent, well-characterized source of this enzyme for in vitro studies. Recombinant production ensures batch-to-batch reproducibility compared with native protein sources, which is critical for reliable experimental outcomes.

For precise details regarding expression system (e.g., mammalian, insect, or E. coli), tag information (e.g., His-tag, Fc-tag), purity percentage, specific activity, formulation buffer, and available quantities, researchers and procurement teams are strongly encouraged to consult the official product datasheet available on the Aladdin Scientific website or contact Aladdin Scientific technical support directly. These specifications may be updated as product lots are refined.

Note: This product is intended for research use only and is not suitable for diagnostic or therapeutic applications in humans.

Recommended Applications

Recombinant Human GALNT9 Protein is designed to support a range of in vitro research applications in glycobiology and related fields. Typical applications for recombinant GalNAc-T family members include:

• In vitro glycosylation assays: Characterizing acceptor substrate specificity of GALNT9 using peptide or glycopeptide substrates and UDP-GalNAc as the sugar donor.
• Enzyme kinetics studies: Measuring Km, Vmax, and catalytic efficiency toward defined substrates.
• Structural biology: As a protein of interest for crystallography or cryo-EM studies of GalNAc-T family members.
• Antibody validation: As a positive control antigen in Western blotting or ELISA-based assays targeting GALNT9.
• Drug discovery: Screening inhibitor or activator compounds targeting O-glycosylation initiation pathways.

Researchers should validate the protein's activity and suitability for their specific assay format. Aladdin Scientific technical support can assist with application-specific recommendations.

Storage, Handling, and Stability

Proper storage and handling of recombinant proteins are essential to maintain activity and integrity. General best-practice guidelines for recombinant enzyme storage apply to Recombinant Human GALNT9 Protein; however, researchers must refer to the specific product datasheet for lot-confirmed storage conditions, as formulation may vary.

• Storage temperature: Typically –20 °C or –80 °C for long-term storage; avoid repeated freeze–thaw cycles, which can reduce enzyme activity.
• Aliquoting: Upon first thaw, it is recommended to prepare single-use aliquots to minimize freeze–thaw damage.
• Working conditions: Keep on ice during experimental setup; avoid prolonged exposure to room temperature.
• Carrier protein: Some formulations include BSA or glycerol as stabilizers — consult the datasheet to confirm compatibility with downstream assays.

If you observe unexpected loss of activity or precipitation, contact Aladdin Scientific customer support for guidance. Storage conditions stated on the official product datasheet supersede any general guidance provided here.

Ordering and Technical Support

Recombinant Human GALNT9 Protein is available from Aladdin Scientific under SKU rp368036. To place an order or request a quotation, visit the Aladdin Scientific website or contact your regional sales representative.

For technical inquiries — including questions about suitability for specific assay formats, recommended positive controls, buffer compatibility, or custom bulk quantities — please reach out to Aladdin Scientific technical support. Our team can provide application notes and datasheet documentation to assist with your experimental planning.

For returns, refunds, or shipping inquiries, please refer to Aladdin Scientific's standard policies available on the website or contact customer service directly.

Frequently asked questions

What is GALNT9 and what does it do biologically?

GALNT9 (Polypeptide N-Acetylgalactosaminyltransferase 9) is a Golgi-localized enzyme belonging to the GalNAc-transferase family. It initiates mucin-type O-linked glycosylation by transferring N-acetylgalactosamine (GalNAc) from UDP-GalNAc onto serine or threonine residues of target proteins, forming the Tn antigen. This modification influences protein folding, stability, cell signaling, and cell–cell interactions.

What are the recommended applications for Recombinant Human GALNT9 Protein (rp368036)?

This recombinant protein is suitable for in vitro glycosylation assays, enzyme kinetics studies, structural biology investigations, use as a positive control antigen in Western blot or ELISA, and inhibitor screening in drug discovery programs. Researchers should confirm suitability for their specific assay format by consulting the product datasheet or contacting Aladdin Scientific technical support.

How should Recombinant Human GALNT9 Protein be stored to maintain activity?

For long-term storage, keep the protein at –20 °C or –80 °C as specified in the product datasheet. Avoid repeated freeze–thaw cycles by preparing single-use aliquots upon first thaw. During experiments, keep the protein on ice and minimize exposure to room temperature. Always refer to the official product datasheet for lot-specific storage recommendations, as formulation details may vary.

Is Recombinant Human GALNT9 Protein suitable for diagnostic or therapeutic use?

No. Recombinant Human GALNT9 Protein (rp368036) from Aladdin Scientific is strictly for research use only (RUO) and is not approved or validated for diagnostic or therapeutic applications in humans or animals. Researchers must comply with all applicable institutional and regulatory guidelines when using this product.

How can I order Recombinant Human GALNT9 Protein or request technical support?

You can order Recombinant Human GALNT9 Protein under SKU rp368036 directly through the Aladdin Scientific website or by contacting your regional sales representative. For technical questions regarding applications, buffer compatibility, or assay optimization, please contact Aladdin Scientific's technical support team, who can provide datasheets and application guidance.

References

1. Bennett, E.P. et al. (2012). Control of mucin-type O-glycosylation: A classification of the polypeptide GalNAc-transferase gene family. Glycobiology, 22(6), 736–756. doi:10.1093/glycob/cwr182
2. Wandall, H.H. et al. (1997). Substrate specificities of three members of the human UDP-N-acetyl-alpha-D-galactosamine:Polypeptide N-acetylgalactosaminyltransferase family. Journal of Biological Chemistry, 272(38), 23503–23514.

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.