Recombinant Human GCFC2 Protein: Properties & Research Use

Recombinant Human GCFC2 Protein is a recombinant form of the human GC-rich sequence DNA-binding factor 2, produced for use in biochemical, cell biology, and transcriptional regulation research applications.

Product Name

Recombinant Human GCFC2 Protein

SKU

rp366434

Gene/Protein Target

GCFC2 (GC-rich sequence DNA-binding factor 2)

Species Origin

Human (Homo sapiens)

Protein Form

Recombinant

Primary Research Area

Transcriptional regulation, nuclear function

Supplier Reference

absea#PP-2023

Background: The GCFC2 Protein

GCFC2, also known as GC-rich sequence DNA-binding factor 2, is a nuclear protein encoded by the GCFC2 gene in humans. It belongs to a family of DNA-binding proteins characterized by their affinity for GC-rich promoter and regulatory sequences. Such proteins play important roles in modulating transcriptional activity by interacting with specific DNA motifs and recruiting co-regulatory complexes.

The GCFC2 protein is thought to participate in the regulation of gene expression programs relevant to cell growth, differentiation, and nuclear organization. Its expression has been detected across multiple human tissues, suggesting a broadly relevant role in mammalian transcriptional control. Research into GCFC2 and related GC-binding factors contributes to a broader understanding of promoter architecture and the mechanisms by which cells fine-tune gene output.

Product Specifications & Quality

The Recombinant Human GCFC2 Protein (SKU: rp366434) offered by Aladdin Scientific is produced using recombinant expression systems to ensure consistency and reproducibility across experiments. Each lot is manufactured under controlled conditions designed to deliver a functional, research-grade protein suitable for a range of biochemical assays.

Researchers should consult the official product datasheet for lot-specific details including expression host, tag information, molecular weight, purity grade, formulation buffer, and recommended storage conditions. Quality control testing is performed to verify protein identity and purity prior to release. The supplier reference for this product is absea#PP-2023.

Note: Specific numerical values for purity percentage, endotoxin levels, and concentration are not reproduced here; please refer to the current product datasheet or contact Aladdin Scientific technical support for the most accurate and up-to-date specifications.

Research Applications

Recombinant Human GCFC2 Protein is suitable for use in a variety of research applications focused on transcriptional regulation and nuclear protein function. Typical applications for recombinant transcription-associated proteins of this class include:

• In vitro binding assays: Electrophoretic mobility shift assays (EMSA) and similar techniques to characterize DNA–protein interactions at GC-rich elements.
• Protein–protein interaction studies: Pull-down assays and co-immunoprecipitation to identify interacting partners within transcriptional complexes.
• Antibody validation: Use as a positive control antigen to validate GCFC2-specific antibodies in Western blot or ELISA formats.
• Structural and biochemical characterization: Biophysical studies to determine binding affinities, conformational properties, and post-translational modification effects.
• Cell-free transcription assays: Reconstitution experiments to assess the functional contribution of GCFC2 to transcriptional output.

Researchers should validate the protein's performance in their specific assay system and consult the datasheet for recommended working concentrations and assay conditions.

Handling, Storage & Reconstitution

Proper handling of recombinant proteins is essential to maintain activity and prevent degradation. General best practices applicable to Recombinant Human GCFC2 Protein include:

• Storage: Store the protein according to the conditions specified in the product datasheet, typically at −20 °C or −80 °C. Avoid repeated freeze–thaw cycles, which can lead to aggregation and loss of activity.
• Reconstitution: If supplied in lyophilized form, reconstitute with the buffer and volume indicated on the datasheet. Allow the vial to equilibrate to room temperature before opening and mix gently by pipetting rather than vortexing.
• Aliquoting: Prepare single-use aliquots upon first thaw to minimize freeze–thaw cycling.
• Working concentrations: Determine optimal concentrations empirically for each assay system; refer to the datasheet for guidance.
• Contamination prevention: Use aseptic technique and sterile consumables when handling the protein to prevent microbial contamination.

Always consult the current lot-specific datasheet for storage temperature, buffer composition, and shelf-life information before use.

Frequently asked questions

What is GCFC2 and why is it relevant to transcription research?

GCFC2 (GC-rich sequence DNA-binding factor 2) is a nuclear protein that binds GC-rich DNA regulatory elements and is implicated in the modulation of gene transcription. Its study is relevant to understanding promoter regulation, gene expression control, and the assembly of transcriptional complexes in human cells.

What applications is Recombinant Human GCFC2 Protein (rp366434) suitable for?

This recombinant protein is suitable for in vitro DNA–protein binding assays (such as EMSA), protein–protein interaction studies, antibody validation as a positive control antigen, and biochemical characterization experiments. Researchers should verify suitability for their specific assay format using the product datasheet as a guide.

How should Recombinant Human GCFC2 Protein be stored to maintain activity?

The protein should be stored at the temperature specified in the lot-specific datasheet, typically −20 °C or −80 °C. Repeated freeze–thaw cycles should be avoided; aliquoting the protein into single-use volumes upon first thaw is strongly recommended to preserve activity.

Where can I find lot-specific data such as purity and concentration for rp366434?

Lot-specific information including purity, concentration, endotoxin levels, and quality control data is provided on the product datasheet supplied with each order. You may also contact Aladdin Scientific technical support or refer to the product page for the most current documentation.

Is Recombinant Human GCFC2 Protein suitable for use in cell-based assays?

While recombinant GCFC2 protein is primarily characterized for in vitro biochemical applications, its suitability for cell-based assays depends on the specific experimental design, delivery method, and assay conditions. Researchers should empirically validate performance in their system and consult the datasheet for recommended applications.

References

1. Product datasheet: Recombinant Human GCFC2 Protein, Aladdin Scientific, SKU rp366434, Supplier Reference absea#PP-2023.
2. UniProt entry for GCFC2 (GC-rich sequence DNA-binding factor 2, Homo sapiens). UniProt Knowledgebase.
3. NCBI Gene entry for GCFC2, Homo sapiens. National Center for Biotechnology Information.

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.