Recombinant Human GNL1 Protein: Research Guide & Applications

Recombinant Human GNL1 Protein is a recombinant form of the human guanine nucleotide-binding protein-like 1, offered by Aladdin Scientific as a high-purity research reagent for studies involving nucleolar function, ribosome biogenesis, and GTPase signaling pathways.

Product Name

Recombinant Human GNL1 Protein

SKU

rp371627

Protein Family

Guanine nucleotide-binding protein-like (GNL) family, MMR1/HSR1 GTPase superfamily

Biological Function

Implicated in nucleolar function, ribosome biogenesis, and intracellular GTP-dependent signaling

Source

Recombinant human protein; expression system to be confirmed with product datasheet

Supplier

Aladdin Scientific

Intended Use

Research use only (RUO); not for diagnostic or therapeutic use

Background and Molecular Biology of GNL1

GNL1 (Guanine Nucleotide-Binding Protein-Like 1), also referred to as HSN2 or Big GTPase, is a member of the MMR1/HSR1 subfamily within the broader family of GTP-binding proteins. This nucleolar GTPase is conserved across eukaryotes and plays roles in fundamental cellular processes including ribosome biogenesis and nucleolar integrity.

GNL1 contains canonical GTP-binding motifs (G1–G5 boxes) characteristic of the P-loop NTPase fold, enabling it to bind and hydrolyze GTP. Its predominantly nucleolar localization suggests it participates in pre-ribosomal RNA processing and the assembly of ribosomal subunits, processes that are tightly linked to cell growth and proliferation.

Dysregulation of nucleolar GTPases, including GNL1, has been associated with altered cellular stress responses and has attracted attention in the context of oncology research, where nucleolar function is frequently perturbed. Researchers utilize recombinant GNL1 to dissect these pathways in biochemical and cell-based assays.

Product Description and Quality Attributes

Aladdin Scientific's Recombinant Human GNL1 Protein (SKU: rp371627) is produced using recombinant DNA technology to deliver a research-grade protein suitable for diverse in vitro applications. The product is referenced under absea catalog number PP-5760, offering researchers a reliable source of GNL1 for mechanistic and functional studies.

Key quality attributes typically assessed for recombinant proteins of this class include:

• Purity: High-purity preparations are standard; specific purity values should be confirmed on the current product datasheet or Certificate of Analysis (CoA).
• Biological Activity: Functional validation (e.g., GTP-binding capacity or activity assays) details should be confirmed with the product datasheet.
• Endotoxin Level: Endotoxin content should be referenced from the product CoA, particularly for cell-based assays.
• Formulation: Buffer composition, additives, and protein concentration are specified on the product datasheet; researchers should consult this document before use.

All quality parameters are verified on a lot-by-lot basis. Researchers are strongly advised to review the current Certificate of Analysis accompanying each shipment.

Handling, Storage, and Stability Guidelines

Proper handling and storage of Recombinant Human GNL1 Protein are critical to maintaining protein integrity, activity, and experimental reproducibility. The following general best-practice guidelines apply; always cross-reference with the product-specific datasheet.

• Upon Receipt: Inspect the shipment immediately. Recombinant proteins are typically shipped on dry ice or with cold packs. Confirm the product arrived in the expected frozen or refrigerated state.
• Short-Term Storage: For immediate use within a few days, store at 2–8 °C. Avoid repeated freeze-thaw cycles, as these degrade protein activity and increase aggregation risk.
• Long-Term Storage: Store at −20 °C or −80 °C as specified on the datasheet. Aliquot the protein into single-use volumes prior to freezing to minimize freeze-thaw cycling.
• Handling: Work on ice when possible. Use low-binding microcentrifuge tubes to minimize protein adsorption to tube walls. Centrifuge briefly before opening to collect contents.
• Carrier Protein: If diluting to very low concentrations, consider adding a carrier protein (e.g., BSA) as specified by your assay requirements, being mindful of potential interference.

Specific storage temperature, recommended diluents, and stability data are provided on the product datasheet and CoA. Refer to these documents as the authoritative source.

Experimental Applications

Recombinant Human GNL1 Protein is suited for a range of in vitro and cell-based research applications. Representative uses include:

• Biochemical Assays: GNL1 can be used in GTP-binding and GTPase activity assays to characterize its enzymatic properties or screen for modulators of its activity.
• Protein–Protein Interaction Studies: Pull-down assays, co-immunoprecipitation validation, and surface plasmon resonance (SPR) or bio-layer interferometry (BLI) experiments can map GNL1 interaction partners within the nucleolar proteome.
• Antibody Validation: Recombinant GNL1 serves as a positive control antigen for validating anti-GNL1 antibodies in Western blot, ELISA, and immunofluorescence applications.
• Structural Studies: High-purity recombinant protein can support X-ray crystallography or cryo-electron microscopy studies aimed at resolving GNL1 structure and its complexes.
• Cell-Free Expression and Reconstitution: Used in reconstitution experiments to study the role of GNL1 in ribosome assembly pathways.
• Drug Discovery: As a target protein in compound screening assays to identify small molecules that modulate GNL1 GTPase activity, relevant to cancer biology research.

Researchers should validate the protein for each specific application and consult the product datasheet for guidance on recommended assay formats and concentrations.

Frequently asked questions

What is GNL1 and why is it important in research?

GNL1 (Guanine Nucleotide-Binding Protein-Like 1) is a nucleolar GTPase conserved in eukaryotes, implicated in ribosome biogenesis and nucleolar integrity. Its dysregulation has been linked to aberrant cell proliferation, making it a relevant target in oncology and cell biology research.

How should I store Recombinant Human GNL1 Protein to maintain its activity?

For short-term use, store at 2–8 °C. For long-term storage, aliquot and freeze at −20 °C or −80 °C as specified on the product datasheet. Avoid repeated freeze-thaw cycles, which can reduce activity and promote protein aggregation.

What applications is Recombinant Human GNL1 Protein (rp371627) validated for?

The protein is suitable for biochemical assays (e.g., GTP-binding and GTPase activity assays), protein interaction studies, antibody validation, structural biology, and drug discovery screening. Specific validated applications are listed on the product datasheet; researchers should confirm suitability for each intended use.

Is Recombinant Human GNL1 Protein suitable for in vivo or clinical use?

No. This product is supplied for research use only (RUO) and is not approved, tested, or intended for diagnostic, therapeutic, or any other clinical application.

Where can I find the Certificate of Analysis (CoA) for my specific lot of rp371627?

The Certificate of Analysis is lot-specific and is typically included with the shipment or accessible via the Aladdin Scientific website by entering the lot number. Contact Aladdin Scientific customer service if you require assistance locating your CoA.

References

1. Leipe DD, Wolf YI, Koonin EV, Aravind L. Classification and evolution of P-loop GTPases and related ATPases. J Mol Biol. 2002;317(1):41-72. doi:10.1006/jmbi.2001.5378
2. Reynaud EG, Andrade MA, Bonneau F, et al. Human Lyst protein recruits clathrin and is involved in endosomal sorting. Traffic. 2005. [GNL family member context — verify applicability to GNL1 specifically]
3. Product page: Recombinant Human GNL1 Protein, SKU rp371627, Aladdin Scientific. absea PP-5760.

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.