Recombinant Human GPR180 Protein: FAQs & Key Facts

Recombinant Human GPR180 Protein is a recombinant form of the human G protein-coupled receptor 180, produced for use in biochemical, cell biology, and drug discovery research applications.

Product Name

Recombinant Human GPR180 Protein

SKU

rp370987

Protein Family

G Protein-Coupled Receptor (GPCR)

Species Origin

Human (Homo sapiens)

Supplier Reference

absea#PP-5187

Recommended Use

Biochemical assays, binding studies, and GPCR research

What Is GPR180 and Why Is It Scientifically Relevant?

GPR180 (G Protein-Coupled Receptor 180) is an orphan receptor belonging to the large and pharmacologically important superfamily of G protein-coupled receptors (GPCRs). GPCRs are seven-transmembrane domain proteins that transduce extracellular signals into intracellular responses and represent one of the most intensively studied drug target classes in biomedical research.

GPR180 is encoded by the GPR180 gene in humans and is expressed in various tissues. As an orphan GPCR, its endogenous ligand has not been fully characterized, making it an active subject of deorphanization studies. Research into GPR180 may have implications for understanding vascular biology, metabolism, and related disease pathways. Recombinant Human GPR180 Protein provides researchers with a defined, reproducible reagent for investigating receptor function, screening potential ligands, and developing assay systems.

Product Specifications and Quality

Recombinant Human GPR180 Protein (SKU: rp370987) is manufactured as a recombinant protein derived from the human GPR180 sequence. The product is referenced under supplier catalog number absea#PP-5187.

Researchers should consult the official Aladdin Scientific product datasheet for the most current and verified specifications, including:

• Expression system (e.g., mammalian, insect, or E. coli host cells)
• Purity grade (typically assessed by SDS-PAGE or HPLC)
• Formulation and buffer composition
• Molecular weight and tag information
• Endotoxin levels

Because transmembrane GPCR proteins present unique challenges in recombinant production, the exact formulation strategy is critical. Please review the product datasheet or contact Aladdin Scientific technical support for confirmed specification details.

Storage, Handling, and Stability

Proper storage and handling of recombinant proteins are essential to maintaining activity and integrity. General best-practice guidelines for recombinant proteins such as GPR180 include:

• Short-term storage: Store at 4 °C if the protein will be used within 1–2 weeks of receipt.
• Long-term storage: Aliquot and store at −20 °C or −80 °C to avoid repeated freeze-thaw cycles, which can degrade protein activity.
• Carrier protein: Addition of a carrier protein such as 0.1% BSA may improve stability during storage.
• Avoid repeated freeze-thaw: Prepare single-use aliquots upon first thaw.

Note: Always refer to the product-specific datasheet for the verified storage conditions and expiry information for SKU rp370987, as conditions may vary by formulation.

Recommended Research Applications

Recombinant Human GPR180 Protein is suitable for a range of in vitro research applications. Common uses for recombinant GPCR proteins of this type include:

• Ligand binding and screening assays: Identifying and characterizing potential endogenous or synthetic ligands for this orphan receptor.
• Antibody production and validation: As an antigen for generating or validating GPR180-specific antibodies.
• Protein–protein interaction studies: Investigating receptor interactions with signaling partners or scaffolding proteins.
• Structural and biophysical studies: Providing a recombinant source for biophysical characterization techniques.
• Drug discovery: Supporting early-stage programs targeting GPCRs.

Suitability for specific applications should be confirmed against the validated applications listed on the official product datasheet. Contact Aladdin Scientific technical support for application-specific guidance.

Ordering and Technical Support

Recombinant Human GPR180 Protein is available from Aladdin Scientific under SKU rp370987. To place an order or request a quotation:

• Visit the Aladdin Scientific website and search by SKU or product name.
• Contact your regional Aladdin Scientific sales representative for bulk pricing, custom formulations, or institutional agreements.
• For technical questions regarding protein activity, assay compatibility, or experimental design, contact Aladdin Scientific technical support directly.

When ordering, ensure you have confirmed the required quantity, preferred formulation, and any specific quality documentation requirements (e.g., Certificate of Analysis, SDS) with your procurement team.

Frequently asked questions

What is GPR180 and what is the significance of its recombinant form?

GPR180 is an orphan G protein-coupled receptor (GPCR) encoded by the human GPR180 gene, with expression reported in multiple tissues. Its endogenous ligand remains to be fully characterized. The recombinant form provides researchers with a defined, reproducible reagent for ligand identification, antibody validation, and drug discovery studies targeting this receptor.

What are the recommended storage conditions for Recombinant Human GPR180 Protein (rp370987)?

As a general guideline for recombinant proteins, short-term storage at 4 °C is suitable if the protein will be used within 1–2 weeks. For long-term storage, aliquot the protein and store at −20 °C or −80 °C to prevent activity loss from repeated freeze-thaw cycles. Always verify storage conditions against the product-specific datasheet for SKU rp370987.

What research applications is Recombinant Human GPR180 Protein validated for?

Typical applications for recombinant GPCR proteins include ligand binding and screening assays, antibody generation and validation, protein-protein interaction studies, and use in drug discovery programs. For the confirmed list of validated applications specific to SKU rp370987, please refer to the official Aladdin Scientific product datasheet or contact technical support.

What expression system is used to produce Recombinant Human GPR180 Protein?

The specific expression system used for Recombinant Human GPR180 Protein (rp370987) is not detailed in the available source material. Expression systems for recombinant GPCR proteins commonly include mammalian, insect (baculovirus), or cell-free systems. Please consult the product datasheet or contact Aladdin Scientific for confirmed expression system details.

How can I order Recombinant Human GPR180 Protein and obtain a Certificate of Analysis?

Recombinant Human GPR180 Protein can be ordered directly from Aladdin Scientific using SKU rp370987 via the website or by contacting a sales representative. A Certificate of Analysis (CoA) is typically available upon request and may be downloadable from the product page using the lot number printed on the vial label.

References

1. Fredriksson R, Lagerstrom MC, Lundin LG, Schioth HB. The G-protein-coupled receptors in the human genome form five main families. Phylogenetic analysis, paralogon groups, and fingerprints. Mol Pharmacol. 2003;63(6):1256-1272. doi:10.1124/mol.63.6.1256
2. Lagerström MC, Schiöth HB. Structural diversity of G protein-coupled receptors and significance for drug discovery. Nat Rev Drug Discov. 2008;7(4):339-357. doi:10.1038/nrd2518
3. Aladdin Scientific. Recombinant Human GPR180 Protein, SKU rp370987. Product Datasheet. Aladdin Scientific; [date not specified].

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.