Recombinant Human HSP90B2P Protein: Usage Guidelines

Recombinant Human HSP90B2P Protein is a research-grade recombinant protein derived from the human HSP90B2P locus, offering scientists a defined reagent for investigating heat shock protein biology, pseudogene-associated expression studies, and related cellular stress response pathways.

Product Name

Recombinant Human HSP90B2P Protein

SKU

rp370897

Species Origin

Human (Homo sapiens)

Protein Family

Heat Shock Protein 90 (HSP90) family

Application

Research use only; suitable for in vitro studies including binding assays, functional studies, and antibody validation

Storage Recommendation

Avoid repeated freeze-thaw cycles; store aliquots at -80°C for long-term stability

Supplier Reference

absea#PP-5110

Biological Background of HSP90B2P

HSP90B2P is a pseudogene locus within the human genome that shares significant sequence homology with members of the Heat Shock Protein 90 (HSP90) family. The HSP90 family comprises highly conserved molecular chaperones that play essential roles in the folding, stabilization, activation, and assembly of a diverse set of client proteins, including kinases, transcription factors, and steroid hormone receptors.

Pseudogenes such as HSP90B2P have historically been considered non-functional genomic relics; however, emerging evidence in molecular biology suggests that some pseudogene-derived transcripts may exert regulatory roles, for example acting as competing endogenous RNAs (ceRNAs) or influencing epigenetic landscapes. Recombinant protein derived from the HSP90B2P locus provides a valuable tool for researchers investigating these emerging functional paradigms.

Understanding the relationship between functional HSP90 paralogs and their pseudogene counterparts is an active area of research with implications in oncology, proteostasis, and stress biology.

Handling and Reconstitution Protocol

Upon receipt, inspect the vial for integrity before opening. Recombinant Human HSP90B2P Protein should be reconstituted according to the specific instructions provided on the Certificate of Analysis (CoA) accompanying each lot. General best-practice guidelines are as follows:

• Centrifugation: Briefly centrifuge the vial (1,000–2,000 × g, 30 seconds) before opening to collect lyophilized material at the bottom of the vial.
• Reconstitution buffer: Use sterile, ultrapure water or the recommended buffer specified on the CoA. Avoid buffers containing strong detergents unless indicated.
• Gentle mixing: Do not vortex. Mix by gently pipetting up and down or by slow rotation to prevent protein denaturation.
• Protein carrier: For dilute solutions, consider adding a carrier protein such as 0.1% BSA (bovine serum albumin) to prevent adsorption to tube walls and maintain stability.
• Aliquoting: Prepare single-use aliquots immediately after reconstitution to minimize freeze-thaw cycles.

All handling should be performed on ice or at 4°C where possible to preserve protein activity and integrity.

Storage Conditions and Stability

Proper storage is critical for maintaining the quality and activity of Recombinant Human HSP90B2P Protein. Adhere to the following recommendations:

• Short-term storage (up to 1 week): Store at 4°C in a suitable stabilizing buffer. Avoid repeated temperature fluctuations.
• Long-term storage: Store at -80°C. Aliquot the reconstituted protein into single-use volumes before freezing to eliminate repeated freeze-thaw cycles, which can cause aggregation and loss of activity.
• Freeze-thaw cycles: Limit to a maximum of 2–3 cycles. Each additional freeze-thaw event risks protein denaturation and degradation.
• Avoid: Storing at -20°C for extended periods (frost-free freezers cause repeated freeze-thaw); exposure to direct light; and storage in non-siliconized tubes, which may cause protein adsorption.

Refer to the lot-specific CoA for confirmed stability data and expiration date. Contact Aladdin Scientific technical support if you observe unexpected precipitation or activity loss.

Research Applications

Recombinant Human HSP90B2P Protein is intended for research use only and is suitable for a range of in vitro laboratory applications, including but not limited to:

• Protein-protein interaction studies: Use as bait or prey in pull-down assays or co-immunoprecipitation setups to identify interacting partners within the HSP90 chaperone network.
• Antibody validation: Serve as a positive control antigen for validating anti-HSP90 or anti-HSP90B2P antibodies by Western blot, ELISA, or immunofluorescence.
• Structural and biophysical studies: Suitable as a reference material in differential scanning fluorimetry (DSF), isothermal titration calorimetry (ITC), or surface plasmon resonance (SPR) experiments to characterize ligand or inhibitor binding.
• Functional chaperone assays: Investigate chaperone-related activities in reconstituted biochemical systems.
• Pseudogene biology research: Explore the potential regulatory or competitive roles of HSP90B2P-derived sequences in cellular contexts.

This product is not intended for diagnostic, therapeutic, or clinical use. Always follow your institution's biosafety guidelines when handling recombinant proteins.

Quality Specifications and Regulatory Notes

Each lot of Recombinant Human HSP90B2P Protein undergoes quality control testing prior to release. Specific purity, concentration, and activity data are documented on the lot-specific Certificate of Analysis (CoA). Researchers should review the CoA upon receipt and retain it for experimental records.

Key quality parameters to verify include:

• Purity: Assessed by SDS-PAGE and/or HPLC; target purity specifications are detailed on the CoA.
• Identity confirmation: Verified by mass spectrometry or N-terminal sequencing where applicable.
• Endotoxin level: Tested by Limulus Amebocyte Lysate (LAL) assay; acceptable thresholds are specified per the CoA.
• Bioactivity: Functional activity data, where available, is provided on the CoA.

This product is manufactured and supplied for research purposes only, in compliance with standard reagent-grade quality management practices. It is not manufactured under GMP conditions and is not suitable for use in humans or animals. The supplier reference for this product is absea#PP-5110. For regulatory compliance questions, contact Aladdin Scientific customer service.

Frequently asked questions

What is HSP90B2P and how does it differ from functional HSP90 proteins?

HSP90B2P is a pseudogene locus in the human genome with significant sequence homology to the HSP90 family of molecular chaperones. Unlike functional HSP90 paralogs (such as HSP90AA1 or HSP90AB1), pseudogene-derived sequences may lack full chaperone activity; however, emerging research suggests HSP90B2P-related transcripts could play regulatory roles in gene expression. Researchers should consult primary literature for the latest functional characterization.

How should I reconstitute and store Recombinant Human HSP90B2P Protein to preserve its activity?

Reconstitute the protein using sterile ultrapure water or the buffer specified on the lot-specific CoA. Mix gently by pipetting; do not vortex. Aliquot immediately into single-use volumes and store at -80°C for long-term use. Limit freeze-thaw cycles to a maximum of 2–3 to prevent denaturation and aggregation.

What applications is Recombinant Human HSP90B2P Protein validated for?

This protein is suitable for research applications including protein-protein interaction assays (pull-down, co-IP), antibody validation by Western blot or ELISA, biophysical studies (DSF, SPR, ITC), and investigation of pseudogene biology. Specific validated applications and quality data are detailed on the CoA provided with each lot. This product is for research use only and is not validated for clinical or diagnostic applications.

Where can I find the Certificate of Analysis (CoA) for my specific lot?

The CoA for each lot of Recombinant Human HSP90B2P Protein is provided with the shipment. You may also contact Aladdin Scientific customer service with your lot number to request a digital copy. Always retain the CoA for your experimental records and audit trail.

Is Recombinant Human HSP90B2P Protein (rp370897) suitable for in vivo or clinical use?

No. This product is strictly for in vitro research use only. It is not manufactured under GMP conditions and has not been evaluated for safety or efficacy in humans or animals. Do not use this product for diagnostic, therapeutic, or any clinical purpose.

References

1. Taipale M, Jarosz DF, Lindquist S. HSP90 at the hub of protein homeostasis: emerging mechanistic insights. Nature Reviews Molecular Cell Biology. 2010;11(7):515-528. doi:10.1038/nrm2918
2. Whitesell L, Lindquist SL. HSP90 and the chaperoning of cancer. Nature Reviews Cancer. 2005;5(10):761-772. doi:10.1038/nrc1716
3. Poliseno L. Pseudogenes: newly discovered players in human cancer. Science Signaling. 2012;5(242):re5. doi:10.1126/scisignal.2002858
4. Absea Biotechnology. Product datasheet: Recombinant Human HSP90B2P Protein, Cat# PP-5110.

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.