Laboratory Protocols & SOPs Library

Schiff's Reagent

· Published 2023/07/27 · Updated 2025/06/09

Recipe

1.Dissolve 5g of basic fuchsin in 900ml of boiling distilled water

2.Cool to approximately 50oC and slowly add 100ml of 1N HCl

3.Cool to approximately 25oC and dissolve 10g of K2S2O5

4.Shake for 3 minutes and incubate in the dark at room temperature for 24 hours

5.Add 5 grams of fine activated charcoal and shake for 3 minutes

6.Filter solution (should be clear)

7.Store at 4oC in a foil covered bottle

Da — when not otherwise indicated, molecular weight units are daltons. Mw — weight-average molecular weight. Mn — number-average molecular weight.

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.

Aladdin Scientific. "Schiff's Reagent" Aladdin Knowledge Base, updated 2025/06/09. https://www.aladdinsci.com/us_ja/faqs/schiffs-reagent-en.html

Was this article helpful? Yes No 3 out 6 found this helpful