In mammalian stable cell line generation, lentiviral/plasmid transduction selection, CRISPR functional screening, and multi-gene co-expression experiments, antibiotic selection systems are used to enrich cells that have successfully integrated or expressed resistance genes.
Topic
Antibiotic selection
Techniques for detecting, quantifying and localizing antigens and antibodies — ELISA, Western blotting, immunohistochemistry and flow cytometry. Below are the protocols, FAQs and technical articles in our knowledge base tagged with this topic.
2 resources · protocols, FAQs & articles
Antibiotics are bioactive molecules produced by microorganisms or obtained via semi-synthetic modification that can inhibit or kill bacteria within defined concentration ranges. In research workflows, their core value lies in providing quantifiable, controllable selection pressure and ...
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Techniques often explored alongside immunological experiments.