Coelenterazine (Coelenterazine, CTZ, CAS 55779-48-1) is a representative marine luciferin that is widely used as the substrate and light-emitting chromophore for multiple marine luciferases and the jellyfish photoprotein (Aequorin). By tuning spectral position, emission intensity, reaction ...
Topic
Bioluminescence
Techniques for detecting, quantifying and localizing antigens and antibodies — ELISA, Western blotting, immunohistochemistry and flow cytometry. Below are the protocols, FAQs and technical articles in our knowledge base tagged with this topic.
2 resources · protocols, FAQs & articles
In firefly luciferase reporter systems, signal quality is jointly determined by substrate accessibility, effective substrate concentration, reaction kinetics, and optical acquisition parameters. Substrate identity alone is rarely the only determinant; without control systems and parameter ...
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Techniques often explored alongside immunological experiments.