This protocol is used to establish a routine workflow for G418 selection, single-clone isolation, clonal expansion, and stable cell line maintenance after CRISPR/Cas9 editing of mammalian cells. It is applicable to CRISPR/Cas9 vectors or co-transfection systems carrying the neomycin resistance ...
Topic
CRISPR
Techniques for detecting, quantifying and localizing antigens and antibodies — ELISA, Western blotting, immunohistochemistry and flow cytometry. Below are the protocols, FAQs and technical articles in our knowledge base tagged with this topic.
2 resources · protocols, FAQs & articles
Human diseases number in the tens of thousands, yet only a fraction have effective treatments—and even fewer can be called “curable.” In many cases, current therapies primarily relieve symptoms or slow progression rather than addressing root causes. In recent years, as CRISPR gene-editing ...
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Techniques often explored alongside immunological experiments.