The electrophoretic mobility shift assay (Electrophoretic Mobility Shift Assay, EMSA) is a classical method for studying protein–nucleic acid interactions. It relies on the observation that binding of a protein to DNA or RNA alters the complex’s net charge, conformation, and hydrodynamic ...
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EMSA
Techniques for detecting, quantifying and localizing antigens and antibodies — ELISA, Western blotting, immunohistochemistry and flow cytometry. Below are the protocols, FAQs and technical articles in our knowledge base tagged with this topic.
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Nucleic acid research spans confirmation of DNA/RNA presence, sequence-level characterization, and mechanistic dissection of interactions between nucleic acids and proteins or other nucleic acids. The choice of experimental method determines evidence type, resolution, throughput, and ...
The electrophoretic mobility shift assay (EMSA), also known as the gel shift assay, is a classic in vitro technique used to detect DNA–protein or RNA–protein interactions under non-denaturing polyacrylamide gel electrophoresis (native PAGE) conditions.
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Techniques often explored alongside immunological experiments.