Affinity chromatography media achieve separation through specific or highly selective interactions between immobilized ligands and target molecules. His-tagged proteins can be captured using Ni²⁺- or Co²⁺-based metal-chelating media, while GST, MBP, and Strep tags correspond to ...
Topic
His Tag
Techniques for detecting, quantifying and localizing antigens and antibodies — ELISA, Western blotting, immunohistochemistry and flow cytometry. Below are the protocols, FAQs and technical articles in our knowledge base tagged with this topic.
3 resources · protocols, FAQs & articles
Tag antibodies are used to recognize exogenous tag sequences in recombinant proteins and are commonly used detection tools in expression validation, protein localization, immunoprecipitation, interaction analysis, and purification process monitoring. Tags such as His, Flag, HA, Myc, V5, GST, ...
His tag grade is a product quality and technical standard level defined around the His (polyhistidine) fusion tag and immobilized metal affinity chromatography (IMAC) systems. This grade focuses on recombinant protein expression, affinity purification, and downstream structural/functional ...
Related topics
Techniques often explored alongside immunological experiments.