Microorganisms can rapidly release excess carbon and generate ATP through acetate-producing pathways, while also activating extracellular acetate into acetyl-CoA. Phosphotransacetylase, acetate kinase, and acetyl-CoA synthetase jointly regulate acetate production, reutilization, and the ...
Topic
Metabolic Engineering
Techniques for detecting, quantifying and localizing antigens and antibodies — ELISA, Western blotting, immunohistochemistry and flow cytometry. Below are the protocols, FAQs and technical articles in our knowledge base tagged with this topic.
2 resources · protocols, FAQs & articles
Glycerol is an important intermediate of lipid catabolism and also a representative non-sugar carbon source in fermentation engineering and metabolic engineering. Unlike classical substrates such as glucose, glycerol must undergo specific transport, phosphorylation, or dehydrogenation steps ...
Related topics
Techniques often explored alongside immunological experiments.