Chymotrypsin (commonly referring to alpha-chymotrypsin) is a canonical pancreatic serine protease. Common laboratory preparations are typically purified from bovine or porcine pancreas and are frequently described commercially as “alpha-chymotrypsin from bovine pancreas” (CAS 9004-07-3). ...
Topic
Peptide Mapping
Techniques for detecting, quantifying and localizing antigens and antibodies — ELISA, Western blotting, immunohistochemistry and flow cytometry. Below are the protocols, FAQs and technical articles in our knowledge base tagged with this topic.
3 resources · protocols, FAQs & articles
Endoproteinase Lys-C (Lys-C) is a lysine-specific serine endopeptidase that preferentially cleaves peptide bonds on the C-terminal side of lysine residues (Lys, K) under mildly alkaline conditions (typically pH 7.0–9.0).
Peptide mapping verifies the integrity of a protein’s primary structure, the correctness of its amino-acid sequence, and potential variants by selectively cleaving the protein and separating/detecting the fragments.
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Techniques often explored alongside immunological experiments.