Step-by-step protocol for preparing, handling, and using Cantharidin (C111020) in PP2A inhibition and cytotoxicity assays. Includes safety and storage guidance.
Topic
Protein Phosphatase
Techniques for detecting, quantifying and localizing antigens and antibodies — ELISA, Western blotting, immunohistochemistry and flow cytometry. Below are the protocols, FAQs and technical articles in our knowledge base tagged with this topic.
3 resources · protocols, FAQs & articles
Protein kinases and phosphatases jointly regulate the phosphorylation states of proteins, thereby affecting cellular signaling, metabolism, proliferation, and stress responses. Phosphorylation or dephosphorylation does not directly equate to protein activation or inactivation, and its effect ...
Protein phosphorylation is one of the most common post-translational modifications in cellular signal transduction and participates in cell proliferation, metabolic regulation, immune activation, DNA damage response, neural plasticity, tumor progression, and other processes. Protein phosphatases ...
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Techniques often explored alongside immunological experiments.