Amide hydrolases participate in amino acid recycling, utilization of nitrogen-containing nutrients, mitochondrial carbon and nitrogen metabolism, urea degradation, and termination of lipid signaling by catalyzing the cleavage of amide bonds. Although different members share the common reaction ...
Topic
Urease
Techniques for detecting, quantifying and localizing antigens and antibodies — ELISA, Western blotting, immunohistochemistry and flow cytometry. Below are the protocols, FAQs and technical articles in our knowledge base tagged with this topic.
2 resources · protocols, FAQs & articles
Urea detection is commonly used for serum/plasma urea nitrogen, urinary urea excretion, cell culture metabolism, fermentation systems, and environmental sample analysis. The urease method depends on enzymatic hydrolysis of urea to produce ammonia and is suitable for clinical automation and ...
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Techniques often explored alongside immunological experiments.