UGGT1 Mouse mAb: FAQs for Research Use

UGGT1 Mouse mAb is a mouse-derived monoclonal antibody targeting UDP-glucose glycoprotein glucosyltransferase 1 (UGGT1), a key sensor enzyme in endoplasmic reticulum glycoprotein quality control, supplied by Aladdin Scientific as catalog Ab351114 in a PBS-only formulation.

Catalog Number

Ab351114

Antibody Type

Mouse Monoclonal Antibody (mAb)

Target Protein

UGGT1 (UDP-glucose glycoprotein glucosyltransferase 1)

Formulation

PBS only (no carrier protein, no azide)

Biological Role of Target

UGGT1 acts as a misfolded-glycoprotein sensor in the ER, re-glucosylating substrates to retain them in the calnexin/calreticulin cycle

Supplier Reference

absea #KC-44774

What Is UGGT1 and Why Is It Studied?

UGGT1 (UDP-glucose:glycoprotein glucosyltransferase 1) is a luminal endoplasmic reticulum (ER) enzyme that functions as the central quality-control sensor for newly synthesized N-glycosylated proteins. It selectively re-glucosylates incompletely folded glycoproteins, tagging them for re-entry into the calnexin (CNX) and calreticulin (CRT) chaperone cycle. This mechanism ensures that only correctly folded glycoproteins exit the ER and proceed through the secretory pathway.

Because UGGT1 is central to ER proteostasis, it is studied in the context of conformational diseases, viral protein maturation, and cancer biology, making a reliable monoclonal antibody an important tool for these research areas.

Product Specifications and Formulation Details

Aladdin Scientific offers UGGT1 Mouse mAb under catalog number Ab351114. Key specification details are as follows:

• Antibody host: Mouse
• Antibody format: Monoclonal antibody (mAb)
• Formulation: PBS only — the antibody is supplied in phosphate-buffered saline without added carrier proteins (such as BSA) or preservatives (such as sodium azide)
• Supplier cross-reference: absea catalog number KC-44774

The PBS-only formulation is advantageous for downstream conjugation, direct labeling, or functional assays where extraneous proteins or azide could interfere with results. Researchers planning long-term storage should consult storage and handling guidelines and consider adding a carrier protein if the antibody will be stored at low concentrations.

Recommended Applications and Usage Guidelines

Mouse monoclonal antibodies against UGGT1 are generally suitable for a range of immunological detection techniques. Common applications for this class of reagent include:

• Western Blot (WB): Detection of UGGT1 in cell lysates or tissue extracts under denaturing conditions.
• Immunoprecipitation (IP): Capture of UGGT1 protein complexes from lysates for co-IP or mass spectrometry studies.
• Immunofluorescence (IF): Visualization of UGGT1 localization within the ER network in fixed cells.
• ELISA: Quantitative or semi-quantitative detection of UGGT1 in suitable sample formats.

Note: Specific validated applications and recommended dilutions for Ab351114 should be confirmed with the product datasheet or by contacting Aladdin Scientific technical support, as application data were not fully detailed in the source material provided.

Storage, Handling, and Stability

Proper storage is critical for maintaining antibody activity. General best-practice recommendations for monoclonal antibodies supplied in PBS-only formulation include:

• Store at -20 °C or -80 °C for long-term storage to preserve activity.
• Avoid repeated freeze-thaw cycles; aliquot the antibody upon first thaw to minimize degradation.
• Short-term storage (up to 1–2 weeks) may be performed at 4 °C.
• Because the formulation contains no azide preservative, adhere strictly to aseptic handling to prevent microbial contamination.
• Do not store in a frost-free freezer, as temperature fluctuations can degrade the antibody.

Always refer to the official product datasheet supplied with Ab351114 for lot-specific storage instructions and expiry information.

Ordering, Availability, and Technical Support

UGGT1 Mouse mAb is available from Aladdin Scientific under SKU Ab351114. To place an order or request a quote:

• Visit the Aladdin Scientific website and search for Ab351114.
• Contact your regional Aladdin Scientific sales representative for bulk pricing, custom packaging, or institutional purchasing agreements.
• For technical questions regarding experimental design, cross-reactivity, or troubleshooting, contact Aladdin Scientific technical support with your lot number and application details.

When ordering, confirm the required quantity and any cold-chain shipping requirements applicable to your region, as antibody products require temperature-controlled delivery to maintain integrity upon arrival.

Frequently asked questions

What is the catalog number for UGGT1 Mouse mAb at Aladdin Scientific?

The catalog number for UGGT1 Mouse mAb at Aladdin Scientific is Ab351114. It is cross-referenced to absea catalog number KC-44774.

What formulation is UGGT1 Mouse mAb (Ab351114) supplied in?

Ab351114 is supplied in PBS only, meaning it does not contain carrier proteins such as BSA or preservatives such as sodium azide. This PBS-only formulation makes it suitable for direct conjugation and applications where azide or extraneous proteins could be problematic.

What biological function does UGGT1 perform in the cell?

UGGT1 is an endoplasmic reticulum enzyme that acts as a quality-control sensor for N-glycosylated proteins. It recognizes incompletely folded glycoproteins and re-glucosylates them, returning them to the calnexin/calreticulin chaperone cycle to allow further folding attempts before ER exit.

How should UGGT1 Mouse mAb be stored to maintain stability?

For long-term storage, keep UGGT1 Mouse mAb at -20 °C or -80 °C. Avoid repeated freeze-thaw cycles by aliquoting the antibody upon first thaw. Short-term storage at 4 °C is acceptable for up to one to two weeks. Always consult the product datasheet for lot-specific guidance.

Which experimental applications is UGGT1 Mouse mAb suitable for?

Mouse monoclonal antibodies targeting UGGT1 are commonly used in Western Blot, immunoprecipitation, immunofluorescence, and ELISA. Researchers should verify validated applications and optimal dilutions for Ab351114 by consulting the official product datasheet or contacting Aladdin Scientific technical support, as specific application data were not detailed in the available source material.

References

1. Aladdin Scientific product listing for UGGT1 Mouse mAb, catalog Ab351114 (absea KC-44774), PBS formulation.
2. Caramelo JJ, Parodi AJ. Getting in and out from calnexin/calreticulin cycles. J Biol Chem. 2008;283(16):10221-10225.
3. Roth RA, Pierce SB. In vivo cross-linking of protein disulfide isomerase to immunoglobulins. Biochemistry. 1987. [General ER quality control reference — flagged for review.]

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.