GFAP Mouse mAb - 一次抗体, specific to GFAP, IgG2a

Cat. No.: Ab179926

グレード＆純度 Carrier Free, ExactAb™, Azide Free, Validated, High Performance, PBS Only, 0.5 mg/mL

Application

WB

Host species

マウス

Reactive species

マウス, 人間

Isotype

Mouse IgG2a

Clone Number

EF03/4D5

Conjugation

Unconjugated

概要

Application
WB: 1/2,000-1/10,000 ; Predicted molecular weight: 50 kDa

仕様

Product Name

GFAP Mouse mAb - 一次抗体, specific to GFAP, IgG2a

同義語

wu:fb34h11 antibody | ALXDRD antibody | cb345 antibody | etID36982.3 antibody | FLJ42474 antibody | FLJ45472 antibody | GFAP antibody | GFAP_HUMAN antibody | gfapl antibody | Glial fibrillary acidic protein antibody | Intermediate filament protein antibod

仕様と純度

Carrier Free,ExactAb™,Azide Free,Validated,High Performance,PBS Only,0.5 mg/mL

宿主種

マウス

特異性

GFAP

免疫原

Recombinant full length protein corresponding to Human GFAP

ポジティブコントロール

WB: Mouse brain tissue lysate

活用

Unconjugated

等級

Azide Free, Carrier Free, ExactAb™, High Performance, PBS Only, Validated

アイソタイプ

IgG2a

ライトチェーン・タイプ

kappa

SDS-PAGE

150 kDa

精製方法

Protein A purified

集中

0.5 mg/mL

Product Line

生物製品

保管と配送

形状

Liquid

保管条件

Store at -20°C,Avoid repeated freezing and thawing

入荷

Ice chest + Ice pads

安定性と保管

Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

画像

Western Blot: GFAP Mouse mAb (Ab179926)

GFAP Mouse mAb (Ab179926) - Western Blot
All lanes: Vinculin Antibody (Ab179926) at 0.5 μg/mL
Samples: Lysate at 20 µg
Secondary: Goat Anti-Mouse IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 50.0 kDa
Observed band size: 45.9 kDa
Exposure time: 66.3 s 

適用＃テキヨウ＃

適用＃テキヨウ＃

Dilution info

WB

1/2,000-1/10,000 ; Predicted molecular weight: 50 kDa

関連ターゲット（人間）

GFAP Tbio Glial fibrillary acidic protein (0 Activities)

Discover Target: GFAP

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Application Protocols

Item-specific

• Tested applications, recommended dilutions, incubation times/temperatures, antigen retrieval details, and positive control samples: Not specified for this item; refer to CoA/Spec Sheet.

General protocol outlines (literature/general knowledge; adjust to your system)

• Western blot: Prepare denatured lysates, run SDS-PAGE, transfer to PVDF/nitrocellulose, block, incubate with primary anti-GFAP mAb (optimize dilution), wash, incubate with species-appropriate secondary, develop via chemiluminescence/fluorescence.
• IHC/IF: Fix tissue/cells, perform antigen retrieval as needed, block, incubate with primary, wash, apply fluorophore- or enzyme-conjugated secondary, counterstain/mount, image.
• IP: Incubate lysate with antibody and Protein A/G support (choice depends on isotype; isotype not specified here), wash, elute, analyze by WB or MS.

These are general templates and not validated for this specific lot. Optimize conditions empirically and consult any available datasheet for item-specific recommendations.

Biological Roles

Target overview: GFAP (glial fibrillary acidic protein)

• Function (literature): GFAP is a type III intermediate filament protein predominantly expressed in astrocytes. It contributes to cytoskeletal architecture, astrocyte mechanical strength, and process extension, and participates in responses to CNS injury and gliosis.
• Isoforms and assembly: GFAP exists as multiple isoforms generated by alternative splicing; monomers polymerize into filaments with vimentin and other IF proteins (literature general knowledge).
• Expression context: High in mature astrocytes; detectable in radial glia, ependymal cells, and certain neural progenitors depending on developmental stage and region.
• Research relevance: Widely used as an astrocyte lineage and activation marker; enables mapping of astroglial networks, neurodevelopmental studies, and characterization of in vitro neural cultures.

This section summarizes general biology of the antigen and does not constitute usage validation for this specific antibody lot. For item-specific performance versus GFAP isoforms and species, consult the CoA/Spec Sheet if available.

Buffer Applications

Item-specific

• Supplied in: PBS Only (from Product Data). Exact pH and salt composition are not specified; refer to CoA/Spec Sheet.

General buffer considerations for antibody workflows (literature/general knowledge)

• Dilution buffers: PBS or TBS at pH ~7.2–7.6. Add 0.05–0.1% Tween-20 for washes in WB/IHC to reduce nonspecific binding (ensure compatibility with your detection system).
• Blocking: Choose blockers appropriate to the assay (e.g., serum, casein, fish gelatin). For carrier-free antibodies, you may add blocking proteins at the application step as needed.
• Storage buffer adjustments: In the absence of azide (this product is Azide Free), maintain sterility; optional 0.02–0.05% sodium azide is commonly used for long-term storage of non–peroxidase assays, but this would conflict with the “Azide Free” attribute and some applications; consider sterile filtration and aliquoting instead.
• Antigen retrieval buffers (IHC): Citrate (pH 6.0) or Tris-EDTA (pH 9.0) are commonly used; conditions must be optimized for GFAP epitopes and tissue fixation (general guidance).

These are general practices; verify compatibility with your protocol and the item’s datasheet.

Green Alternatives

Context: For antibody reagents, sustainability focuses on formulation, shipping, and experimental design rather than substituting a greener solvent.

• Azide Free: Eliminates azide-related aquatic toxicity and hazardous waste concerns; consider sterile technique or low-biotoxin preservatives if compatible with your assay to maintain shelf-life without azide.
• Carrier Free: Reduces non-biodegradable protein additives and simplifies downstream purification of conjugates, decreasing resource use in cleanup steps.
• Shipping & storage: Product ships with ice packs (from Product Data). Consolidating orders and minimizing dry-ice shipments can lower cold-chain environmental impact.

Greener practice suggestions (general/literature)

• Miniaturize immunoassays (micro-volume staining, capillary-based WB) to reduce reagent consumption and waste.
• Use reusable staining chambers and low-plastic workflows; select non-toxic detergents and block reagents when possible.
• Buffer selection: PBS prepared in-house from concentrates can reduce packaging; validate pH/ionic strength to maintain antibody stability.

No direct “alternative chemical” is applicable for a target-specific mAb; sustainability gains are best achieved via workflow optimization and careful inventory management to avoid repeated freeze–thaw cycles and wastage.

Pharmaceutical Uses

Not applicable as a finished pharmaceutical. This product is designated for research use only.

General notes (non-clinical, formulation/manufacturing context)

• Monoclonal antibodies can serve as analytical reagents in bioprocess development (e.g., QC immunoassays) or as affinity ligands in purification studies. However, no such use is specified for this item.
• No pharmacopeial monograph, GMP status, or excipient role is provided. Do not use in human or veterinary applications.

Physical Properties

Item-specific

• Molecular weight (exact), molecular formula, extinction coefficient, concentration, isotype, and formulation details beyond “PBS Only”: Not specified for this item; refer to CoA/Spec Sheet.

General/literature values for IgG-class antibodies

• Typical molecular weight: ~150 kDa for full-length IgG; ~50 kDa (heavy chain) and ~25 kDa (light chain) under reducing SDS-PAGE (literature).
• Physical state: Usually supplied as an aqueous solution or lyophilizate; this item indicates “PBS Only,” suggesting phosphate-buffered saline vehicle; presence of other excipients not specified.
• pI: Monoclonal antibodies commonly exhibit pI ~6–9 depending on sequence (literature; item-specific pI not specified).
• Solubility: Readily soluble in aqueous buffers (PBS, TBS) at neutral pH (literature).
• Stability factors: Temperature, freeze–thaw cycling, pH, and ionic strength strongly influence aggregation and activity (general guidance).

Note: Do not construe the above as product specifications. For exact values (protein concentration, buffer composition, and any stabilizers) consult the CoA/Spec Sheet.

Quality and Grades

Item-specific quality attributes (from Product Data)

• Carrier Free: No protein carriers (e.g., BSA) added. Facilitates conjugation (enzymes, fluorophores) and minimizes background in sensitive assays.
• ExactAb™: Branded quality designation; typically denotes stringent lot-to-lot consistency and accuracy of concentration/identity (interpretation; consult datasheet for specifics).
• Azide Free: No sodium azide preservative. Important for compatibility with live cells, primary neuron/astrocyte cultures, and peroxidase-based assays (azide inhibits HRP).
• Validated; High Performance: Indicates in-house validation and performance benchmarking; specific validated applications and metrics are not provided here.
• PBS Only: Supplied in phosphate-buffered saline; absence of other excipients/stabilizers is implied but not item-spec confirmed beyond “PBS Only”.

Implications for experimental design (general guidance)

• Without carriers and azide, the antibody is well-suited for custom labeling and functional assays but may require stricter aseptic handling to prevent microbial growth.
• Lot documentation: For exact concentration, purity profile, residuals, and isotype/clone information, consult the CoA/Spec Sheet.

Note: No additional numeric specifications (e.g., concentration, endotoxin) are provided; do not assume values beyond the statements above.

Reaction and Applications

As a monoclonal antibody, this product is used in immunoassays rather than chemical synthesis.

Item-specific (from Product Data)

• No specific tested applications or protocols provided. Validated/High Performance is stated but not tied to particular methods.

General research applications for anti-GFAP monoclonal antibodies (literature/general knowledge)

• Immunocytochemistry/Immunofluorescence (ICC/IF): Detection of GFAP in cultured astrocytes or neural stem cell derivatives; often requires permeabilization and appropriate secondary antibodies.
• Immunohistochemistry (IHC): Visualization of astrocytic intermediate filaments in fixed brain or spinal cord sections; antigen retrieval conditions depend on fixation and tissue processing.
• Western blot (WB): Denatured GFAP detection; common apparent band ~49–55 kDa (isoform- and species-dependent; literature typical). Optimize sample prep to preserve intermediate filament integrity.
• Immunoprecipitation (IP)/Co-IP: Enrichment of GFAP from cell/tissue lysates for downstream analysis.
• Flow cytometry (less common): Possible in permeabilized cells for intracellular GFAP.

Note: The above are general use-cases; they are not confirmed for this specific lot. Optimize antibody dilution, incubation time, and detection systems empirically, and consult the CoA/Spec Sheet for any item-specific validation data.

Reaction Conditions

Classical chemical reaction conditions are not applicable to monoclonal antibody usage.

General assay conditions (literature/general knowledge)

• Temperature: Many immunoassays incubate at room temperature or 4°C; elevated temperatures can accelerate binding but may increase background.
• Time: Primary antibody incubations range from 1–2 h at RT to overnight at 4°C depending on assay and antigen abundance.
• Buffers: PBS or TBS with defined blockers and detergents as appropriate to the assay.

These points are provided as general guidance, not item-specific recommendations. For this particular product, no tested conditions are specified; consult the CoA/Spec Sheet and optimize empirically.

Safety and Handling

Item-specific hazard information

• GHS Classification, Signal Word, H/P Statements, and Pictograms: Not specified for this item; refer to the SDS.
• Research Use: For research use only (as provided).

General safety guidance for protein antibodies (literature/general knowledge)

• Handle using good laboratory practice. Wear appropriate PPE: lab coat, gloves, safety glasses. Avoid ingestion, inhalation of aerosols, and skin/eye contact.
• Biological origin considerations: Although purified and intended for research, treat as potentially bioactive material. Avoid microbial contamination; work aseptically when appropriate.
• Incompatibilities: Avoid strong oxidizers and extremes of pH that can denature proteins. Avoid foaming and vigorous vortexing that can induce aggregation.
• First aid (general): In case of skin/eye contact, rinse with water. If inhaled, move to fresh air. If ingested, rinse mouth. Seek medical attention if symptoms persist. Defer to SDS for authoritative measures.
• Thermal sensitivity: Follow storage guidance strictly; repeated freeze–thaw can degrade performance.

Always consult the product’s SDS and institutional EHS policies for definitive hazard classification and emergency procedures.

Solvent Selection

This product is a protein antibody formulated for aqueous use; organic solvent selection is generally not applicable.

Item-specific

• Buffer system: PBS Only (from Product Data). This typically supports stability at neutral pH and isotonic ionic strength. Exact composition (phosphate concentration, NaCl, pH) is not specified; refer to CoA/Spec Sheet.

General guidance (literature)

• Preferred matrices: PBS or TBS for dilutions; add protein stabilizers (e.g., 0.1–1% inert protein or 0.05% nonionic surfactant) only if compatible with your assay. For this item, “Carrier Free” implies no carriers are included; adding them is user-dependent.
• Avoid: High percentages of organic solvents (acetone, acetonitrile, methanol, DMSO) which can denature or precipitate antibodies. If conjugation requires organic cosolvent, keep below denaturation thresholds and validate.
• pH considerations: Maintain near-neutral pH (6.8–7.6) for most storage/assay conditions unless your protocol defines otherwise.

Select buffers based on downstream application (IHC, IF, WB, IP), antigen retrieval, and detection system compatibility rather than classical solvent polarity comparisons.

Storage and Reconstitution

Item-specific (from Product Data)

• Storage: Store at -20°C. Avoid repeated freezing and thawing.
• Shipping: Ice chest + ice pads.
• Formulation: PBS Only; Carrier Free; Azide Free. Exact PBS composition and protein concentration: Not specified for this item; refer to CoA/Spec Sheet.

General handling guidance (literature/general knowledge)

• Upon receipt: Inspect for container integrity and temperature. Briefly centrifuge to collect liquid from cap/threads.
• Aliquoting: Divide into single-use aliquots to prevent freeze–thaw cycles; store at recommended temperature. For short-term use (days to a week), 4°C storage may be acceptable if sterility is maintained; validate stability in your lab.
• Reconstitution: If supplied lyophilized (not specified here), reconstitute in sterile PBS or assay buffer to the desired concentration, mixing gently to avoid foaming. Allow solution to equilibrate on ice before use.
• Sterility: Being azide-free, maintain aseptic technique; consider 0.22 µm sterile filtration after reconstitution/preparation if compatible with your application.

Always refer to the CoA/Spec Sheet for lot-specific storage buffer, concentration, and any additional stabilizers or recommended storage nuances.

Structure and Identity

Item-specific (from Product Data)

• Product: GFAP Mouse mAb (SKU: Ab179926)
• Type: Mouse monoclonal antibody (mAb) targeting GFAP
• Grade/Attributes: Carrier Free; ExactAb™; Azide Free; Validated; High Performance; PBS Only
• CAS, CID, InChIKey, SMILES: Not specified for this item; refer to CoA/Spec Sheet.

General antibody structure (literature/general knowledge)

• Typical architecture: Immunoglobulin G (IgG)-class monoclonal with two identical heavy chains and two identical light chains forming a Y‑shaped heterotetramer stabilized by disulfide bonds.
• Approximate mass: ~150 kDa for intact IgG (literature typical); actual mass/isotype for this item: Not specified for this item; refer to CoA/Spec Sheet.
• Antigen: GFAP (glial fibrillary acidic protein), an intermediate filament protein predominantly expressed in astrocytes.
• 2D description in words: Two Fab arms (antigen-binding fragments) with variable domains that recognize GFAP epitopes; one Fc stem (constant domains) mediating effector functions (class/isotype-specific; not specified here).

Synthetic Utility

Not typically applicable. As a target-specific monoclonal antibody, this product is not a synthetic building block or reagent for chemical transformations.

For research uses, see the Reaction & Applications and Application Protocols sections for immunoassay-oriented guidance.

Target Specificity

Item-specific (from Product Data/Product Name)

• Antigen/Target: GFAP (glial fibrillary acidic protein)
• Host: Mouse monoclonal antibody (mAb)
• Clone, isotype/subclass, epitope mapping, species reactivity, and cross-reactivity: Not specified for this item; refer to CoA/Spec Sheet.

No additional specificity data are provided. Users should confirm reactivity in their system and consult the datasheet when available.

よくある質問

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Carrier Free mean?

Carrier Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (GFAP Mouse mAb); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.