グレード＆純度BioReagent?BioReagent grade — tested suitable for life-science and molecular-biology use. Use for cell culture, assays, and biochemical work needing biological compatibility.
Determine the necessary mass, volume, or concentration for preparing a solution.
Dilution Calculator
Determine the dilution needed to prepare a stock solution.
Reconstitution Calculator
レビュー
顧客レビュー
Application Protocols
No manufacturer-tested application protocols are provided in the Product Data for this item.
General pointers (not item-specific; verify with CoA/SDS and institutional SOPs)
Motility test (concept): Prepare semi-solid deeps, stab inoculate with a straight needle, incubate under appropriate conditions, and assess diffusion of growth away from the stab line.
Fermentation readout: If using indicators (not specified for this item), interpret color change and turbidity according to the indicator’s response and organism’s metabolism.
For detailed, validated protocols (e.g., inoculum size, incubation time/temperature, interpretation criteria), consult recognized microbiology manuals and your laboratory’s SOPs.
Biological Roles
Context for glucose and semi-solid media (literature/general)
Glucose: Central carbon and energy source funneled through glycolysis to pyruvate, then to the TCA cycle or fermentation pathways. Supports ATP generation and biosynthesis (e.g., via pentose phosphate pathway for NADPH and ribose-5-phosphate).
Semi-solid matrix: Restricts convective mixing, permitting diffusion-limited gradients of oxygen and nutrients. This environment enables phenotypic assays (e.g., motility, chemotaxis) and selective growth of microaerophiles.
Acidification: In organisms that ferment glucose, acid end-products can lower local pH; when indicators are present (not specified for this item), color changes provide readouts of carbohydrate utilization.
Biofilm initiation: Reduced shear in semi-solid systems can favor initial adhesion and microcolony formation studies (where components are compatible), though dedicated biofilm media are typically used for structured biofilms.
Relevance to lab workflows
Phenotypic differentiation: Enables discrimination of strains by motility and carbohydrate metabolism.
Maintenance: Provides baseline nutrition without the full complexity of enriched broths, which can be advantageous for maintaining selective pressures or observing subtle phenotypes.
Item-specific biological performance metrics (e.g., growth-promotion organisms, indicator systems): Not specified for this item; refer to CoA/Spec Sheet.
Buffer Applications
This product is not a buffer system; it is a semi-solid microbiological medium.
General notes (literature/practice)
Many agar media include buffering salts to maintain near-neutral pH during growth; exact buffer chemistry for this item is not specified.
If pH control is critical (e.g., acid production from glucose metabolism), consider supplementing with appropriate indicators or using defined media with known buffering capacity. Validate any modifications with growth-promotion testing.
Item-specific buffer composition, target pH, and capacity: Not specified for this item; refer to CoA/Spec Sheet.
Green Alternatives
Sustainability considerations for culture media (literature/general)
Renewable matrix: Agar is derived from red seaweed (renewable biomass). Compared with petroleum-derived gelling agents, this generally reduces fossil feedstock dependence.
Energy use: Autoclaving dominates energy consumption. Batch planning (full autoclave loads), using insulated media bottles, and minimizing re-sterilization events can reduce energy usage.
Waste minimization: Prepare only the volume needed; semi-solid deeps in screw-cap tubes can reduce disposable plastic plate usage in some assays.
Alternative matrices and trade-offs (general)
Gellan gum: Comparable or stronger gels at lower concentration; clearer gels beneficial for imaging. May require specific cations for optimal gelation; supply chain differs from agar.
Plant-based pectins/carrageenan blends: Potential options but often less thermally reversible or with different gel strengths; compatibility with microbial growth varies.
Comparison snapshot (general, not item-specific)
Agar (this medium): Robust thermal hysteresis (sets ~35 °C, melts ~85 °C), widely validated in microbiology; renewable; straightforward handling.
Gellan: Lower use levels, excellent clarity; may need Ca2+/Mg2+; cost can be higher.
Note: No formulation substitutions are implied for this catalog item. Adjustments should be validated by growth-promotion testing.
Pharmaceutical Uses
No clinical or therapeutic claims. For research and quality control contexts only.
General industry context (literature/practice; not item-specific)
Microbiological QC: Semi-solid agar media are used in environmental monitoring, motility tests, and maintenance cultures within non-sterile and sterile pharmaceutical manufacturing QC labs, consistent with compendial methods where applicable.
Method suitability: Media used for compendial testing (e.g., USP/EP micro tests) require demonstrated growth promotion and, where relevant, selectivity. Whether this product meets a compendial specification is not specified.
Item-specific pharmacopeial status, compliance, or GPT outcomes: Not specified for this item; consult CoA/Spec Sheet and site-specific validation.
Physical Properties
Item-specific facts (from Product Data)
Appearance: Not specified for this item; refer to CoA/Spec Sheet.
Storage: Room temperature (per Product Data).
General/literature values and characteristics for semi-solid agar media (not item-specific)
Physical state (dry): Typically a free-flowing beige to off-white powder before hydration; becomes a translucent gel after hydration/sterilization. Not specified for this item.
Gelling characteristics (agar, literature):
Gelation (setting) temperature: ~32–40 °C after cooling from melt.
Melting temperature: ~80–90 °C (agar gels melt well above their setting point), enabling heat sterilization and pourability.
Water content and ash: Not specified for this item.
Solubility: Dispersible in water with heating to near boiling; insoluble in most organic solvents (literature). Not specified for this item.
pH (of prepared medium): Not specified for this item; typical non-selective nutrient media target ~pH 6.8–7.4 (literature), contingent on buffering salts and peptone content.
Osmolality/ionic strength: Not specified for this item.
Refractive index/optical clarity: Dependent on formulation and sterilization; not specified for this item.
Note: Numerical specifications for this catalog item are not provided; consult CoA/Spec Sheet for exact physical specifications and preparation parameters.
Quality and Grades
Item-specific facts (from Product Data)
Grade: BioReagent.
What BioReagent grade generally implies (literature/industry practice)
BioReagent grade materials are suitable for biological research applications where consistent performance in cell culture or microbiology is required. While not a pharmacopeial grade, they are produced with attention to bioburden, performance characteristics (e.g., growth promotion), and lot-to-lot consistency.
For culture media, quality is typically demonstrated via growth promotion testing (GPT) with representative microorganisms, gel strength/penetration for semi-solid formulations, pH after preparation, clarity, and sterility after autoclaving.
Item-specific clarifications
Exact formulation, performance criteria, and growth-promotion organisms for this item: Not specified for this item; refer to CoA/Spec Sheet.
Stabilizers/indicators: Not specified for this item.
Practical guidance
Verify lot-specific CoA for: recommended preparation mass per liter, target pH after sterilization, gel firmness (semi-solid percent agar), and GPT results.
Maintain consistent water quality (e.g., ASTM Type II or better) and autoclave profile to minimize variability across lots and between labs.
Reaction and Applications
This product is a semi-solid culture medium used for microbiological workflows rather than chemical synthesis.
Applications (literature/practice; not item-specific)
Bacterial motility testing: Semi-solid agar allows differentiation of motile vs non-motile organisms by diffuse growth away from the stab line.
Aerotolerance and gradient studies: The semi-solid matrix can establish oxygen gradients useful for examining microaerophiles and facultative anaerobes.
Carbohydrate utilization: Inclusion of glucose provides a readily metabolizable carbon source to support growth and to couple with indicators (if added) for fermentation/acidification readouts.
Maintenance and transport: Semi-solid deeps can be used for short-term maintenance or transport of bacterial cultures with reduced desiccation risk compared to plates.
Practical tips
Gas exchange: Use caps that allow limited gas exchange when appropriate; excessive tightening can create unintended anaerobiosis.
Inoculation: Stab inoculate with a straight needle to a consistent depth to compare motility between strains.
Reading results: Incubate at the organism’s optimal temperature; interpret after defined intervals to avoid overgrowth leading to ambiguous results.
Item-specific parameters (inoculum size, incubation temperature/time, indicators): Not specified for this item; consult CoA/Spec Sheet and institutional SOPs.
Reaction Conditions
This section is adapted to preparation/usage conditions for culture media (general literature guidance; not item-specific). Always follow the CoA/Spec Sheet when provided.
Typical preparation workflow (general)
Hydration: Disperse the specified mass of dehydrated medium into purified water (volume per CoA). Allow to pre-soak 5–10 min to minimize clumping.
Heating: Bring to a vigorous boil with stirring until the agar is fully dissolved and the solution is clear.
Sterilization: Autoclave at 121 °C for 15–20 min (liquid cycle). Do not overtighten caps.
Cooling and pouring: Cool to ~45–50 °C, then dispense into sterile tubes (for deeps) or plates as required. For semi-solid motility deeps, maintain consistent fill volume and depth for reproducibility.
Supplements: Heat-labile additives (e.g., antibiotics, indicators) should be sterile-filtered and added aseptically after cooling to ~45–50 °C.
Incubation (general)
Temperature/time: Incubate inoculated media at organism-appropriate conditions (e.g., 25–42 °C for many bacteria) and read at defined time points to assess motility/fermentation.
Item-specific mass-per-liter, target pH after sterilization, and gel firmness: Not specified for this item; refer to CoA/Spec Sheet.
Safety and Handling
Item-specific facts (from Product Data)
GHS classification, signal word, pictograms, H-statements: Not specified for this item; refer to the SDS for authoritative safety information.
Shipping: Normal (per Product Data).
Storage: Room temperature (per Product Data).
Research use: For research use only.
General safety guidance (literature/practice; not item-specific)
Expected hazards: Most dehydrated culture media are considered non-hazardous under GHS; however, dust may cause mechanical irritation to eyes/respiratory tract. Some formulations may contain irritants; always check the SDS.
PPE: Use lab coat, safety glasses, and appropriate gloves. Avoid generating dust/aerosols. Employ local exhaust when weighing powders.
Handling: Rehydrate carefully to avoid clumping; add powder slowly to vigorously stirred cold/room-temperature water before heating. Do not ingest or inhale. Treat used media as biohazardous if exposed to microorganisms.
Incompatibilities: Strong oxidizers and strong acids/bases may degrade organic components. Avoid prolonged exposure to high humidity; hygroscopic uptake can alter performance.
First aid (overview): If inhaled, move to fresh air; if eye contact, rinse cautiously with water; if skin contact, wash with soap/water; if ingested, rinse mouth. Seek medical advice as needed. Follow SDS.
Sterilization: Autoclave per standard practices (e.g., 121 °C). Use heat-resistant containers; do not seal tightly during autoclaving to prevent overpressure.
Waste and decontamination
Dispose of spent/contaminated media as regulated biological waste following institutional biosafety procedures.
Solvent Selection
Not a solvent; this is a dehydrated microbiological medium intended for reconstitution in water.
General guidance (literature/practice)
Reconstitution medium: Use purified/deionized water (microbiological grade) for hydration. Avoid high-ionic-strength buffers unless specified by the formulation, as they can alter gelation and organism motility.
pH adjustment: If directed by the CoA, adjust with dilute acid/base prior to sterilization; otherwise, do not modify.
Additives: Antibiotics, indicators, or supplements (e.g., TTC, dyes) are typically sterile-filtered and added after autoclaving and cooling to ~45–50 °C to preserve activity.
Comparison notes (selection among media types)
Semi-solid vs solid agar: Semi-solid (lower agar concentration) is preferred for motility tests and microaerophilic growth support; solid plates (1.5–2% agar) are preferred for colony isolation. Liquid broths are used for rapid biomass expansion or enrichment.
General guidance (literature/practice; not item-specific)
Storage of dehydrated powder: Keep tightly closed in original container, in a cool, dry place away from humidity and direct light. Reseal promptly after use. Avoid long-term exposure to air to prevent moisture uptake and caking.
Shelf life: Use by the labeled date on the container. Lot-specific expiry: Refer to product label/CoA.
Reconstitution: Hydrate with purified/deionized water per CoA mass-per-liter instructions (not specified for this item). Mix thoroughly, heat to dissolve, and sterilize by autoclaving.
Prepared medium: Store poured, sterile deeps or plates at 2–8 °C, protected from dehydration (e.g., sealed bags for plates, tight caps for tubes). Warm to room temperature before use to avoid condensation artifacts.
Freeze–thaw: Do not freeze prepared agar media; freezing disrupts gel structure and performance.
Item-specific pH targets, mass-per-liter, and hold times: Not specified for this item; refer to CoA/Spec Sheet.
Research use only: Not for human or animal therapeutic use.
Structure and Identity
Item-specific facts (from Product Data)
Product type: Glucose Semi-Solid Agar (formulated microbiological medium; mixture)
Grade: BioReagent
Single-compound identifiers (CAS, InChIKey, SMILES, MF, MW): Not applicable to formulated media; not specified for this item.
General description (literature/typical)
Composition concept: Semi-solid agar media are typically agar-based gels at reduced gelling agent concentration (commonly ~0.2–0.5% w/v agar) supplemented with a utilizable carbon source (here, glucose). Exact formulation and percentages are not specified for this item; refer to CoA/Spec Sheet.
Structural features: Heterogeneous mixture of a polysaccharide gelling matrix (agar; primarily agarose and agaropectin polymers of galactose) plus low-molecular-weight nutrients (e.g., D-glucose). No defined 2D structure exists for the product as a whole.
Functional intent: Provides a semi-solid, low-strength gel that permits limited bacterial motility while supporting growth via glucose metabolism.
How to visualize the “structure” (descriptive)
Matrix: Interpenetrating agarose helices forming a hydrated network that immobilizes bulk water but allows diffusion of small molecules (e.g., glucose, metabolites, dyes, and gases).
Synthetic Utility
Not a synthetic building block or reagent. As a formulated semi-solid growth medium, it does not participate in named organic reactions and is not intended for chemical synthesis.
If a microbiology workflow intersects with synthesis (general)
Strain engineering: Semi-solid media can aid in colony selection/motility phenotyping prior to scaling engineered strains for biocatalysis in separate liquid media.
Screening: Phenotypic screens for carbohydrate utilization can inform metabolic engineering but are distinct from synthetic organic chemistry operations.
Target Specificity
Not an antibody or affinity reagent; target specificity does not apply.
Item-specific antigen/epitope/clone/isotype: Not applicable and not specified for this item.
Products are supplied to verified businesses, institutions, and qualified professionals for research and development use only. Not for use in humans, animals, diagnosis, or therapy.
