GPR15L, Agonist of GPR15;Agonist of MRGPRX1;Agonist of MRGPRX2, CAS No.rp174164, Agonist of GPR15;Agonist of MRGPRX1;Agonist of MRGPRX2

CAS: rp174164 Cat. No.: rp174164
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グレード&純度 Moligand™ ? Moligand™ — Aladdin's line of ligands and bioactive small molecules. Use for receptor, pathway, and binding studies needing defined small-molecule tools.
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サイズ
USA
ドイツ (EU)*
価格
個数
500μg
rp174164-500μg
US製・受注生産 · 2~4週間 ·
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$2,399.90
1mg
rp174164-1mg
US製・受注生産 · 2~4週間 ·
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$3,999.90
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このグレードを選ぶ理由

Moligand™ Moligand™ ベースライン干渉を最小限に抑える必要がある、感度の高いクロマトグラフィーおよび分析ワークフロー向け。

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保管・配送

Room temperature に出荷。正確な仕様については、ロット固有の COA をご確認ください。

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品質書類

SDS、COA、データシート、仕様書をダウンロードできます。ロット固有の COA はロット番号検索で入手可能です。

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クロマトグラフィー、有機合成、クロスカップリング反応に関する 0 件の査読済み論文で引用されています。

仕様

Product Name
GPR15L, Agonist of GPR15;Agonist of MRGPRX1;Agonist of MRGPRX2, CAS No.rp174164
同義語
antimicrobial peptide-57;colon-derived SUSD2 binding factor
等級
Moligand™
仕様と純度
Moligand™
アクションタイプ
AGONIST
作用メカニズム
Agonist of GPR15;Agonist of MRGPRX1;Agonist of MRGPRX2
CAS
rp174164
分子タイプ
ペプチド
Product Line
生物製品
保管と配送
保管条件
Room temperature

ドキュメント

📋 安全データシート(SDS)

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✅ 分析証明書(COA)

ロット固有の品質データ。ロット番号を入力して、正確な COA を取得してください。

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📊 データシート

製品仕様と用途のクイックリファレンス概要。

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このグレードの完全な品質特性と合格基準。

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詳細データ

関連ターゲット(人間)
GPR15 Tbio G-protein coupled receptor 15 (0 Activities)
Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)
MRGPRX1 Tchem Mas-related G-protein coupled receptor member X1 (0 Activities)
Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)
MRGPRX2 Tchem Mas-related G-protein coupled receptor member X2 (0 Activities)
Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)
証明書(CoA、COO、BSE/TSEと分析図)
C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:
遺伝情報
Alternate Namesantimicrobial peptide-57;colon-derived SUSD2 binding factor
Reference
  • 1. Kinetics and inhibition of recombinant human cystathionine gamma-lyase. Toward the rational control of transsulfuration., The Journal of biological chemistry, Steegborn, C C and 7 more authors.
  • 2. Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences., Proceedings of the National Academy of Sciences of the United States of America, Strausberg, Robert L RL and 83 more authors.
  • 3. Genomic basis of cystathioninuria (MIM 219500) revealed by multiple mutations in cystathionine gamma-lyase (CTH)., Human genetics, Wang, Jian J and Hegele, Robert A RA.
  • 4. Cloning and nucleotide sequence of human liver cDNA encoding for cystathionine gamma-lyase., Biochemical and biophysical research communications, Lu, Y Y, O'Dowd, B F BF, Orrego, H H and Israel, Y Y.
  • 5. Single nucleotide polymorphism in CTH associated with variation in plasma homocysteine concentration., Clinical genetics, Wang, J J, Huff, A M AM, Spence, J D JD and Hegele, R A RA.
  • 6. Cystathionine gamma-lyase overexpression inhibits cell proliferation via a H2S-dependent modulation of ERK1/2 phosphorylation and p21Cip/WAK-1., The Journal of biological chemistry, Yang, Guangdong G, Cao, Kun K, Wu, Lingyun L and Wang, Rui R.
  • 7. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)., Genome research, Gerhard, Daniela S DS and 115 more authors.
  • 8. Towards a proteome-scale map of the human protein-protein interaction network., Nature, Rual, Jean-François JF and 37 more authors.
  • 9. The DNA sequence and biological annotation of human chromosome 1., Nature, Gregory, S G SG and 178 more authors.
  • 10. Polymorphisms in one-carbon metabolism and trans-sulfuration pathway genes and susceptibility to bladder cancer., International journal of cancer, Moore, Lee E LE and 14 more authors. more
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レビュー

顧客レビュー

Application Protocols

No tested-application data (e.g., WB, IHC, IF, FC) or protocol specifics are provided in the Product Data for this SKU. The following is general guidance for deploying a ligand in receptor assays (non-item-specific):

  • Stock preparation:
    • Dissolve at high concentration (e.g., 10–50 mM in DMSO for small molecules, or in sterile buffer for peptide-like ligands) based on pilot solubility tests.
    • Aliquot to avoid repeated freeze–thaw; label with lot, concentration, and preparation date.
  • Cell-based functional assays:
    • Plate validated receptor-expressing cells; equilibrate in assay buffer (e.g., HBSS/HEPES, 0.1% BSA).
    • Perform serial dilutions to generate concentration–response curves; include solvent controls.
    • Readouts may include Ca2+ flux, cAMP, β-arrestin, or reporter assays; optimize incubation time and temperature.
  • Binding assays:
    • Use membranes or intact cells; consider radioligand/fluorescent tracer displacement formats.
    • Optimize nonspecific binding conditions and washing steps to obtain robust signal-to-noise.
  • Data analysis:
    • Fit concentration–response data with appropriate models (e.g., four-parameter logistic), report EC50/IC50 with confidence intervals and replicate statistics.

For item-specific directions, consult the CoA/Spec Sheet and SDS.

Biological Roles

Context (general, literature): “GPR15L” denotes an endogenous ligand identified for the G protein–coupled receptor GPR15. In the literature, GPR15L has been characterized as a chemokine-like, epithelial-derived peptide with roles in immune cell trafficking. The specific composition of this catalog item is not disclosed; therefore, the following describes biological context rather than item-specific claims.

  • Receptor interaction: Acts as a natural ligand for GPR15, a class A GPCR. Reported to activate receptor signaling pathways typical of GPCRs (e.g., G protein and/or β-arrestin pathways; literature).
  • Cellular effects (literature context): In model systems, GPR15L engagement can promote chemotaxis of GPR15-expressing T cells and modulate immune homing behaviors.
  • Expression context: GPR15 is found on subsets of T cells and other immune cells; GPR15L is reported to be secreted by epithelial tissues (literature), contributing to localized cell recruitment.
  • Biophysical considerations: If peptide-like, GPR15L activity can be sensitive to proteases, ionic strength, and pH; binding/activation may demonstrate species-dependent potency.

Usage in research (non-clinical):

  • Tool ligand for receptor pharmacology, signaling pathway dissection, and screening of analogs or antagonists.

Important: The above reflects general scientific literature and is not a statement about the exact composition, potency, or sequence/structure of this SKU. Consult the CoA/Spec Sheet and generate empirical data in your assay system.

Buffer Applications

Item-specific buffer recommendations are not provided; the following are general practices for GPCR ligand handling in biochemical or cell-based assays.

  • Stock solutions:
    • Prepare concentrated stocks in DMSO (common practice) or in aqueous buffer if the material is peptide-like and water-soluble. Verify solubility and stability.
  • Working buffers (examples; literature context):
    • HBSS or PBS with calcium/magnesium for cell assays; often supplemented with 0.1% BSA to reduce nonspecific adsorption.
    • HEPES-buffered saline (10–25 mM HEPES, pH 7.2–7.4) to stabilize pH during incubations outside CO2.
    • For peptide-like ligands: consider low concentrations of carrier protein (0.05–0.1% BSA) and protease inhibitors where appropriate.
  • pH range:
    • Most GPCR assays operate near physiological pH (7.2–7.4); verify receptor-specific requirements.
  • Filtration and sterility:
    • Filter aqueous working solutions (0.22 µm) to remove particulates; prepare fresh daily to limit degradation.
  • Adsorption mitigation:
    • Use low-binding plasticware; pre-condition wells with buffer containing BSA or a non-ionic surfactant compatible with the assay.

Note: Because this SKU lacks item-specific composition/sequence/structure in the Product Data, finalize buffer composition and storage conditions empirically and by referencing the CoA/Spec Sheet and SDS.

Green Alternatives

Sustainability considerations for ligand handling focus on solvent choice, waste minimization, and cold-chain avoidance when possible.

Greener practices (general; not item-specific):

  • Prefer aqueous buffers when solubility and stability allow; reduce reliance on polar aprotic solvents.
  • If DMSO is required, minimize final assay percentage (≤0.1–0.2% v/v where feasible) and consolidate stock solutions to reduce plastic consumables.
  • Use microscale assay formats (384/1536-well) to cut solvent volumes and waste.
  • Investigate bio-based or lower-toxicity co-solvents (e.g., glycerol, propylene glycol, PEG-400) for intermediate dilutions if assay-compatible.

Trade-offs (balanced view):

  • Aqueous buffers enhance safety/environmental profile but may compromise solubility for hydrophobic ligands.
  • DMSO offers superior solvency but carries worker-exposure considerations and higher environmental impact per liter of waste.

Illustrative comparison (general literature):

  • Water/buffer: greenest; requires intrinsic solubility; potential adsorption losses for peptide-like ligands unless carrier is used.
  • DMSO: high solvency; low volatility; can facilitate dermal absorption; handle with PPE.
  • Ethanol: renewable sources possible; volatile organic emissions; lower solvency vs DMSO.

Operational tips:

  • Prepare high-concentration master stocks to minimize total solvent use.
  • Plan batchwise plate preparation to avoid repeated freeze–thaw cycles and reduce discarded leftovers.

Note: Specific greener substitutions depend on the actual structure and solubility profile; consult the CoA and pilot tests.

Pharmaceutical Uses

No pharmacopeial status or excipient role is provided for this SKU. As listed, it is a research-use-only Moligand™ reagent and is not intended for human or veterinary use.

Research and development context (general, non-clinical):

  • Assay system controls: May serve as a tool ligand or control in preclinical screening, receptor profiling, and SAR studies focused on GPR15 pathway biology.
  • Formulation studies (in vitro only): Solubility/stability assessments in various vehicles (buffers, DMSO, co-solvents) to support assay development.
  • Reference material: Potential internal reference for method development (e.g., LC/MS method suitability, plate uniformity testing) where permitted by internal QA.

Not included/Not applicable for this item:

  • No claims for therapeutic efficacy, dosage forms, clinical indications, or GMP manufacturing are made or implied.

Compliance reminder:

  • Use strictly in research laboratories under appropriate institutional approvals. Review the CoA/Spec Sheet and SDS for batch-specific information before use.
Physical Properties

Item-specific physicochemical values are not provided in the Product Data for this Moligand™ listing.

  • Appearance: Not specified for this item; refer to CoA/Spec Sheet.
  • Molecular Weight: Not specified for this item; refer to CoA/Spec Sheet.
  • Molecular Formula: Not specified for this item; refer to CoA/Spec Sheet.
  • Melting Point / Boiling Point: Not specified for this item; refer to CoA/Spec Sheet.
  • Density / Refractive Index: Not specified for this item; refer to CoA/Spec Sheet.
  • LogP / cLogP: Not specified for this item; refer to CoA/Spec Sheet.
  • pKa: Not specified for this item; refer to CoA/Spec Sheet.
  • UV-Vis characteristics: Not specified for this item; refer to CoA/Spec Sheet.
  • Solubility: Not specified for this item; refer to CoA/Spec Sheet.

Practical notes (general, literature-informed for GPCR ligands; not item-specific):

  • Many screening ligands are supplied as solids or films, intended for dissolution in DMSO to make high-concentration stocks (e.g., 10–50 mM) before dilution into assay buffer.
  • Peptide-like ligands (literature context) may dissolve in aqueous buffers (e.g., PBS) with 0.1% carrier protein (BSA) to minimize adsorption; salts (acetate, TFA) can influence apparent solubility and pH.
  • Always verify solubility and stability experimentally for your specific batch using the CoA/Spec Sheet and small-scale trial dissolutions.

Note: All item-specific physical-property values should be taken from the product’s CoA/Spec Sheet and/or SDS when available.

Quality and Grades
  • Grade/Purity: Moligand™ (as listed in Product Data)

What Moligand™ typically implies (general description; not item-specific specifications):

  • Curated ligand quality suitable for discovery screening, assay development, and target validation studies.
  • Typical QC for ligand-grade materials often includes identity confirmation (e.g., HRMS/ESI-MS, NMR where applicable) and chromatographic purity assessment (e.g., HPLC/UPLC). Exact methods and acceptance criteria vary by compound class.

Item-specific details for this SKU:

  • Purity percentage, residual solvents, counterions/salts, water content, metal/halide limits, optical rotation, and UV cutoffs: Not specified for this item; refer to CoA/Spec Sheet.
  • Stabilizers or additives: Not specified for this item; refer to CoA/Spec Sheet.

Practical guidance for users:

  • Request and review the CoA for the exact batch you receive to confirm identity, purity, and any stabilizers/counterions that may influence solubility or bioassay behavior.
  • For screening campaigns, document plate maps and replicate performance; if using multiple lots, evaluate lot-to-lot consistency.
  • Where possible, perform incoming QC (e.g., quick LC/MS check) to verify integrity after shipping and upon long-term storage.

Note: Do not treat the above as specifications—only the CoA/Spec Sheet governs the item-specific quality attributes for this product.

Reaction and Applications

This product is positioned as a Moligand™ research ligand rather than a synthetic reagent; thus, its primary applications are biological/biochemical assays rather than chemical transformations.

Applicable research uses (general, non-clinical):

  • Target engagement studies: Evaluate binding or activity toward the GPR15 receptor in cell-based or membrane-preparation assays (e.g., reporter gene readouts, cAMP/Ca2+ flux, β-arrestin recruitment).
  • Assay development and validation: Use as a reference or probe ligand to establish dynamic range, EC50/IC50 windows, and Z′-factor in high-content or high-throughput screening.
  • Mechanistic profiling: Map concentration–response, potency, efficacy (agonism/antagonism/biased signaling) under varied buffer conditions, temperature, or cofactor presence.
  • Selectivity panels: Cross-screen against a GPCR panel to assess specificity; generate SAR hypotheses for follow-up chemistry.

Not typically used for chemical synthesis:

  • Named organic reactions (e.g., cross-couplings, condensations) are not relevant here. For synthetic chemistry contexts, choose appropriate building blocks or catalysts instead.

Practical notes:

  • Standardize solvent, plate format, and incubation times to reduce inter-plate variability.
  • Include solvent-only and known-ligand controls. Implement counter-screens (e.g., cytotoxicity, reporter interference) to exclude artifacts.

Manufacturer Applications: The catalog lists no additional application text for this SKU; the above points provide general, expandable use-cases consistent with Moligand™ ligands.

Reaction Conditions

Not typically applicable. This product is a research ligand for biological assays rather than a reagent for organic synthesis.

General notes for assay execution (not chemical reaction conditions):

  • Temperature: Many GPCR functional assays run at 20–37°C; binding assays may be conducted at 4–25°C depending on kinetics.
  • Incubation times: 15–90 minutes are common for endpoint functional readouts; adjust to reach steady-state response.
  • Solvent tolerance: Keep DMSO or other organic co-solvents at or below the assay’s validated threshold (commonly ≤0.1–0.5% v/v).
  • Controls: Include known agonist/antagonist controls (if available), solvent controls, and counter-screens for reporter interference.

Because no item-specific synthetic transformations are relevant, conventional parameters such as catalysts, equivalents, and thermal profiles are not provided. Refer to the CoA/Spec Sheet and your internal assay SOPs for definitive guidance.

Safety and Handling

The Product Data for this SKU does not list hazard classifications.

  • Signal word: Not specified for this item; refer to SDS.
  • GHS classification and pictograms: Not specified for this item; refer to SDS.
  • H-statements/P-statements: Not specified for this item; refer to SDS.

General laboratory precautions (not a substitute for SDS):

  • Handle in a chemical fume hood when weighing, dissolving, or handling powders/lyophilized materials to avoid inhalation of particulates or aerosols.
  • Wear appropriate PPE: lab coat, safety glasses, and nitrile gloves. Avoid skin and eye contact; wash thoroughly after handling.
  • Prevent cross-contamination by using clean, dedicated tools. Avoid repeated vial openings in humid rooms to limit moisture uptake for hygroscopic materials.
  • If using DMSO or other polar aprotic solvents for stock preparation, avoid skin exposure (DMSO facilitates dermal absorption of solutes).

First aid (general):

  • Inhalation: Move to fresh air; seek medical advice if symptoms occur.
  • Skin/eyes: Rinse with water for several minutes; remove contaminated clothing; obtain medical attention if irritation persists.
  • Ingestion: Rinse mouth; do not induce vomiting; seek medical attention.

Stability and incompatibilities:

  • No item-specific data provided. As a precaution, protect from strong oxidizers, bases/acids, and extreme temperatures. Consult the product SDS and CoA for authoritative guidance.

Note: For research use only; not for diagnostic or therapeutic applications.

Solvent Selection

Since item-specific solubility data are not provided, select solvents based on general practices for GPCR ligands and screening compounds.

  • Primary stock solvent choices (general):
    • DMSO: Most common for screening stocks; broad solubilizing power; compatible with most cell-based and biochemical assays at ≤0.1–0.5% v/v final.
    • Water or buffer (e.g., PBS, HEPES): If the ligand is peptide-like or supplied as a salt with high aqueous solubility. Carrier protein (e.g., 0.1% BSA) can mitigate adsorption.
    • Ethanol/MeOH: Secondary options when DMSO is undesirable; confirm assay tolerance.
  • Polarity class & dielectric (general context):
    • DMSO (ε ≈ 47, literature) and water/buffers (ε ≈ 80, literature) support polar/ionic ligands. Ethanol (ε ≈ 24, literature) suits moderately polar analytes.
  • Practical tips:
    • Prepare concentrated master stocks (e.g., 10–50 mM in DMSO; literature practice) and dilute into assay buffer, ensuring final solvent ≤ assay’s tolerated limit.
    • Pre-wet plasticware with buffer containing inert protein or surfactant if adsorption is observed.
    • Filter aqueous working solutions (0.22 µm) if compatible; avoid filtering concentrated DMSO stocks.

Small comparison (general, literature):

  • DMSO: best solubilizer; potential cell membrane effects at >0.5%.
  • EtOH: volatile; lower solvency than DMSO but often acceptable ≤1%.
  • Aqueous buffer: highest biological compatibility; requires intrinsic compound solubility.

Always confirm with the item’s CoA/Spec Sheet and run a small test dissolution.

Storage and Reconstitution
  • Storage Conditions (as provided): Room temperature.
  • Shipped In: Not specified for this item; refer to CoA/Spec Sheet.
  • Appearance: Not specified for this item; refer to CoA/Spec Sheet.

Reconstitution guidance (general; verify for your lot):

  • If supplied as a solid or film: equilibrate to room temperature before opening to prevent moisture condensation. Dissolve in an appropriate solvent (commonly DMSO for small molecules, or sterile aqueous buffer for peptide-like ligands) following a small-scale solubility check.
  • Prepare concentrated master stocks (e.g., 10–50 mM in DMSO, or mg/mL in buffer for peptide-like materials) and sterile-filter aqueous solutions when compatible.
  • Avoid repeated freeze–thaw by aliquoting. Record concentration, solvent, and date.

Working solution handling:

  • Dilute stocks into assay buffer just before use. Maintain final organic solvent content within assay tolerance (often ≤0.1–0.5% v/v).
  • Protect from prolonged light exposure if chromophoric groups are suspected; use amber vials when in doubt.

Longer-term practices (general recommendations; user to validate):

  • Unopened vials per Product Data may be kept at room temperature. For prepared solutions, many labs store aliquots at −20°C (or 2–8°C for short term) to limit degradation—confirm stability for your lot via the CoA/Spec Sheet.

Note: For research use only. The CoA and SDS for your batch are the authoritative sources for storage, stability, and reconstitution specifics.

Structure and Identity

Brief overview: GPR15L is supplied as a Moligand™ research ligand intended for GPCR-related studies. Item-specific structural identifiers are largely not provided in the listing.

  • Product Name: GPR15L
  • SKU: rp174164
  • Category: Small molecules and compound libraries (小分子和化合物库)
  • CAS: rp174164 (catalog placeholder)
  • InChIKey: 221808 (as provided in Product Data)
  • SMILES: Not specified for this item; refer to CoA/Spec Sheet.
  • Molecular Formula: Not specified for this item; refer to CoA/Spec Sheet.
  • Molecular Weight: Not specified for this item; refer to CoA/Spec Sheet.

Structural features (general knowledge):

  • The designation “GPR15L” commonly refers in the literature to an endogenous ligand for the GPR15 GPCR (chemokine-like/peptide-like in origin). However, the precise chemical form, salt, sequence, or small-molecule surrogate used in this product is not disclosed in the Product Data.
  • Without an item-specific structure or sequence, stereochemistry, ring systems, and functional groups cannot be enumerated for this catalog entry.

2D structural description (not item-specific):

  • If the supplied material is a peptide-like ligand (literature context), it would feature a backbone of amide linkages and ionizable side chains typical of cationic antimicrobial/chemokine-like peptides. If it is a small-molecule surrogate, heteroatoms (N/O/S) and polar pharmacophore elements enabling GPCR engagement would be expected. Please consult the item’s CoA/Spec Sheet for the definitive structure applicable to this SKU.
Synthetic Utility

This SKU is offered as a Moligand™ research ligand rather than a synthetic building block or reagent. As such, classical synthetic utility (e.g., participation in named organic reactions, protecting-group strategies, or cross-couplings) is not typically applicable.

For chemists considering derivatization or analog generation (general guidance, not item-specific):

  • If the material is peptide-like: analog design may proceed via solid-phase peptide synthesis (SPPS), residue substitution scans (Ala-scan), N/C-terminal capping, backbone cyclization, or noncanonical residue incorporation to probe stability and receptor bias.
  • If the material is a small-molecule surrogate: SAR can be pursued by modifying heteroatom substituents, ring systems, or linker motifs that engage orthosteric/allosteric pockets of GPR15 (guided by docking or structural biology where available in literature).

Limitations:

  • Without item-specific structure, no reliable retrosynthetic analysis or reaction conditions can be proposed. Any modification program should start from authenticated structure data and confirmed analytical characterization.

Recommendation:

  • Obtain or determine the exact structure (CoA/Spec Sheet) before planning chemical transformations. Maintain analytical fingerprints (LC/MS, NMR) throughout any derivatization campaign.
Target Specificity

Only Product Data may be used here. For this SKU, no target-binding specifications or biological performance data are provided.

  • Intended target (catalog context): GPR15 (G protein–coupled receptor 15) — inferred from the product name; however, item-specific binding constants, agonist/antagonist mode, and species selectivity are not provided in the listing.
  • Affinity/potency (Ki/EC50/IC50): Not specified for this item; refer to CoA/Spec Sheet.
  • Selectivity profile: Not specified for this item; refer to CoA/Spec Sheet.
  • Assay validation data: Not specified for this item; refer to CoA/Spec Sheet.

Recommendation:

  • Establish target specificity empirically in your assay system (e.g., receptor-overexpressing vs. parental cells, use of selective antagonists, or genetic knockout/knockdown controls) and document performance alongside lot-specific CoA information.

よくある質問

What is Moligand??
Moligand? is one of Aladdin Scientific's premium product lines, formulated for high-purity applications across multiple workflows. Compared to standard grades in the same workflow, Moligand? products typically offer tighter specifications, more rigorous QC, and stronger lot-to-lot consistency.
What documentation is provided?
Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.
How should this product be stored?
Store at room temperature.

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