GRB2 Mouse mAb - 一次抗体, specific to GRB2, Mouse IgG1

Cat. No.: Ab106387

GRADE & PURITY Validated, ExactAb™, 4.2 mg/mL

Application

WB

Host species

マウス

Reactive species

マウス, 人間, 助言

Isotype

Mouse IgG1

Clone Number

5G3-E7-F11

Conjugation

Unconjugated

概要

Mouse anti Human GRB2 Antibody, Monoclonal (5G3-E7-F11), could be used for WB and so on .
Application:
WB: 1/500-1/1000
Protein Function
Adapter protein that provides a critical link between cell surface growth factor receptors and the Ras signaling pathway.
Isoform GRB3-3 does not bind to phosphorylated epidermal growth factor receptor (EGFR) but inhibits EGF-induced transactivation of a RAS-responsive element. Isoform GRB3-3 acts as a dominant negative protein over GRB2 and by suppressing proliferative signals, may trigger active programmed cell death.

Specifications

Product Name

GRB2 Mouse mAb - 一次抗体, specific to GRB2, Mouse IgG1

同義語

GRB2 | ASH | Growth factor receptor-bound protein 2 | Adapter protein GRB2 | Protein Ash | SH2/SH3 adapter GRB2 | Abundant SRC homology antibody | Adapter protein GRB2 antibody | ASH antibody | Ash protein antibody | EGFRBP GRB2 antibody | Epidermal growt

仕様と純度

ExactAb™, Validated, 4.2 mg/mL

宿主種

マウス

特異性

GRB2

免疫原

Recombinant protein of human GRB2 (AA 1-217).

ポジティブコントロール

WB：K562, Raji, Jurkat, Ramos, C6, NIH/3T3, HeLa, MCF7 and A431 cell lysates.

活用

Unconjugated

等級

ExactAb™, Validated

アイソタイプ

Mouse IgG1

SDS-PAGE

150 kDa

精製方法

Protein G purified

集中

4.2 mg/mL

保管と配送

形状

Liquid

保管条件

Store at -20°C,Avoid repeated freezing and thawing

入荷

Ice chest + Ice pads

安定性と保管

Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

画像

Western Blot: GRB2 Mouse mAb (Ab106387)

GRB2 Mouse mAb (Ab106387) - Western Blot
All lanes: GRB2 Mouse mAb (Ab106387) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040)at 1/20000 dilution

Predicted band size: 25 kDa
Observed band size: 25 kDa
Exposure time: 7.2 s

適用＃テキヨウ＃

• 適用＃テキヨウ＃: Dilution info
• WB:

  1/500-1/1000

関連ターゲット（人間）

GRB2 Tchem Growth factor receptor-bound protein 2 (0 Activities)

Discover Target: GRB2

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

作用メカニズム

よくある質問

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Validated mean?

Validated indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (GRB2 Mouse mAb); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.