GRADE & PURITYValidated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.1.0 mg/mL
ExactAb™, Validated, 1.0 mg/mL ExactAb™,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
概要
Mouse anti Human HDAC4 Antibody, Monoclonal antibody(4A3-H7-C6), could be used for WB, IP and so on. Application: WB: 1/500-1/1000 IP: 1/20 Protein Function Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Involved in muscle maturation via its interaction with the myocyte enhancer factors such as MEF2A, MEF2C and MEF2D. Post-translational Phosphorylated by CaMK4 at Ser-246, Ser-467 and Ser-632. Phosphorylation at other residues is required for the interaction with 14-3-3. Sumoylation on Lys-559 is promoted by the E3 SUMO-protein ligase RANBP2, and prevented by phosphorylation by CaMK4.
Specifications
Product Name
HDAC4 Mouse mAb - 一次抗体, specific to HDAC4, Mouse IgG2a
WB: Jurkat, NIH/3T3 and C6 cell lysates. IP: HeLa lysates.
活用
Unconjugated
等級
ExactAb™, Validated
アイソタイプ
Mouse IgG2a
ライトチェーン・タイプ
kappa
SDS-PAGE
150 kDa
精製方法
Protein G purified
集中
1.0 mg/mL
保管と配送
形状
Liquid
保管条件
Store at -20°C,Avoid repeated freezing and thawing
入荷
Ice chest + Ice pads
安定性と保管
Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
画像
HDAC4 Mouse mAb (Ab107057) - Western Blot All lanes: HDAC4 Mouse mAb (Ab107057) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution
Predicted band size: 119 kDa Observed band size: 134 kDa Exposure time: 60.0 s
HDAC4 Mouse mAb (Ab107057) - Western Blot All lanes: HDAC4 Mouse mAb (Ab107057) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution
Predicted band size: 119 kDa Observed band size: 134 kDa Exposure time: 30.0 s
HDAC4 Mouse mAb (Ab107057) - Western Blot Immunoprecipitation analysis of HDAC4 in HeLa lysates using HDAC4 Mouse mAb (Ab107057)
適用＃テキヨウ＃
適用＃テキヨウ＃
Dilution info
WB
1/500-1/1000
IP
1/20
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
1.Yong Ling, Yangyang Li, Rui Zhu, Jianqiang Qian, Ji Liu, Weijie Gao, Chi Meng, Jiefei Miao, Biao Xiong, Xiaodong Qiu, Changchun Ling, Hong Dai, Yanan Zhang. (2019) Hydroxamic Acid Derivatives of β-Carboline/Hydroxycinnamic Acid Hybrids Inducing Apoptosis and Autophagy through the PI3K/Akt/mTOR Pathways. JOURNAL OF NATURAL PRODUCTS, [PMID:31120744][10.1021/acs.jnatprod.8b00843]
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レビュー
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よくある質問
How should this product be stored?
Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.
What does ExactAb? mean?
ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.
What does Validated mean?
Validated indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.
How is this product shipped?
This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.
What documentation is provided?
Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.
Which species reactivity has this antibody been validated against?
Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (HDAC4 Mouse mAb); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.
Need help choosing the grade?
Our grade selection guide covers purity, stabilizer status, and application suitability for all variants in our catalog.
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