High-efficiency debris remover - BioReagent

Cat. No.: H1508752

GRADE & PURITY BioReagent

Synonyms

Debris Removal Solution | Debris Depletion Reagent | Cell Debris Eliminator | Single-Cell Suspension Debris Remover | Tissue Dissociation Debris Clearing Agent | Debris Removal Buffer | Dead Cell Removal Solution

Storage

Store at 2-8°C

Shipped In

Wet ice

Application

Cell Culture, Cell Separation

Overview

This product is a highly efficient and gentle debris removal reagent specifically designed for single-cell suspensions prepared from various animal organs (such as brain, liver, spleen, kidney, lung, tumor tissue, etc.) via enzymatic digestion or mechanical grinding.Through a unique gradient separation principle, it can rapidly and selectively sediment cell debris, dead cells, and impurities, while retaining highly viable and pure target cells (e.g., primary cells, immune cells), thereby greatly improving the success rate and data quality of downstream experiments such as flow cytometry, single-cell sequencing, and cell culture.

Technical Principle

This product is a specially formulated isotonic and sterile solution with precisely optimized density and viscosity.During centrifugation, the solution forms a stable density barrier within the medium.Viable and intact target cells are pelleted by centrifugation, while cell debris and other impurities remain in the supernatant.Pure live cells can be separated from debris by centrifugation, and the target cells are obtained by discarding the supernatant, achieving efficient purification.

Product Advantages

1. Efficient Debris Removal, Enhanced Purity: Effectively removes over 95% of cell debris and impurities, significantly reducing background noise, resulting in clearer flow cytometry plots and higher yield and purity in cell sorting.
2. Cell-Friendly, Viability Protection: Optimized formulation ensures a gentle separation process with minimal damage to live cells, maximally preserving their original state and biological functions, and ensuring the reliability of downstream culture and functional assays.
3. Broad Compatibility, Flexible Application: Suitable for different tissue types from various animal sources (mouse, rat, human, etc.), and demonstrates excellent separation performance for cell suspensions derived from tissues with different characteristics.
4. Simple Operation, Time-Saving: Classic centrifugation-based separation method, no need for expensive specialized equipment. The procedure is simple and fast, completing the entire purification process within 15 minutes, significantly improving experimental efficiency.
5. Ready-to-Use, Stable and Reliable: The product is a ready-to-use solution with strict quality control, minimal batch-to-batch variation, and stable performance, ensuring the reproducibility of experimental results.

Application Scenarios

1. Preprocessing before flow cytometry analysis: Removes debris, reduces non-specific background, and improves sorting accuracy and resolution.
2. Single-cell RNA sequencing sample preparation: Obtains high-purity, high-viability single-cell suspensions, which is a critical prerequisite for obtaining high-quality sequencing data.
3. Primary cell culture and functional studies: Purified cells are easier to attach and grow, reducing interference from impurities on cell viability.
4. Functional assays such as cell killing, proliferation, and apoptosis: Ensures the accuracy and reproducibility of experimental results.
5. Isolation and enrichment of rare cells (e.g., stem cells, circulating tumor cells).

Protocol

1. Sample Preparation: Resuspend the dissociated single-cell suspension in PBS to a density of 1–5×10⁶ cells/mL, and mix with the debris removal reagent at a 1:1 volume ratio. If debris is abundant, increase the proportion of the debris removal reagent.
2. Centrifugation: Centrifuge at 400–800×g for 10 minutes (enable full acceleration and deceleration of the centrifuge). After centrifugation, debris is enriched in the upper layer, while intact cells are pelleted at the bottom.
3. Cell Collection: Carefully aspirate and discard the supernatant, then resuspend the cell pellet in PBS.
4. Washing and Resuspension: Wash 1–2 times with PBS or culture medium to obtain high-purity, high-viability target cells for downstream experiments.

Notes

Debris levels may vary depending on the tissue and cell type. For optimal debris removal efficiency, pre-experiments can be performed to adjust the volume mixing ratio of the debris removal reagent to the cell suspension for better results.

Specifications

Synonyms

Debris Removal Solution | Debris Depletion Reagent | Cell Debris Eliminator | Single-Cell Suspension Debris Remover | Tissue Dissociation Debris Clearing Agent | Debris Removal Buffer | Dead Cell Removal Solution

Specifications & Purity

BioReagent

Stability And Storage

Store at 2-8℃ long term (12 months).

Storage

Store at 2-8°C

Shipped In

Wet ice

This product requires cold chain shipping. Ground and other economy services are not available.

Grade

BioReagent