HLA E Antibody - 一次抗体, specific to HLA-E, Rabbit IgG

Cat. No.: Ab108192

GRADE & PURITY Validated, ExactAb™, 1.0 mg/mL

Application

WB

Host species

ウサギ

Reactive species

マウス, 人間, 助言

Isotype

Rabbit IgG

Conjugation

Unconjugated

概要

Rabbit anti Human HLA E Antibody, Polyclonal, could be used for WB and so on.
Application:
WB: 1/500-1/2000
Relevance
HLA E belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. HLA E binds a restricted subset of peptides derived from the leader peptides of other class I molecules.

Specifications

Product Name

HLA E Antibody - 一次抗体, specific to HLA-E, Rabbit IgG

同義語

EA1.2 antibody | EA2.1 antibody | HLA 6.2 antibody | HLA class I histocompatibility antigen alpha chain E antibody | HLA class I histocompatibility antigen E alpha chain precursorantibody | HLA class I histocompatibility antigen E alpha chain precursor an

仕様と純度

ExactAb™, Validated, 1.0 mg/mL

宿主種

ウサギ

特異性

HLA-E

免疫原

A synthetic peptide derived from human HLA-E

ポジティブコントロール

WB: A431, Jurkat, THP-1, K562, Raji, NIH/3T3 and C6 cell lysates.

活用

Unconjugated

等級

ExactAb™, Validated

アイソタイプ

Rabbit IgG

SDS-PAGE

150 kDa

精製方法

Immunogen affinity purified

集中

1.0 mg/mL

保管と配送

形状

Liquid

保管条件

Store at -20°C,Avoid repeated freezing and thawing

入荷

Ice chest + Ice pads

安定性と保管

Store at 4°C short term (1-2 weeks). Store at -20°C long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

画像

Western Blot: HLA E Antibody (Ab108192)

HLA E Antibody (Ab108192) - Western Blot
All lanes: HLA E Antibody (Ab108192) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 40 kDa
Observed band size: 35 kDa
Exposure time: 7.0 s

適用＃テキヨウ＃

• 適用＃テキヨウ＃: Dilution info
• WB:

  1/500-1/2000;

よくある質問

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Validated mean?

Validated indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (HLA E Antibody); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.