Hsp27 Mouse mAb - 一次抗体, specific to Hsp27

Cat. No.: Ab210504

GRADE & PURITY Animal Free, Carrier Free, ExactAb™, Azide Free, Validated, High Performance, PBS Only, ≥95%(SDS-PAGE), 1.0 mg/mL

Application

ELISA, Flow Cytometry, WB

Host species

マウス

Reactive species

人間

Isotype

Mouse IgG2b

Clone Number

CD04/1E2

Conjugation

Unconjugated

概要

Mouse anti Human Hsp27 Antibody, clone CD04/1E2, could be used for WB, Flow Cytometry, ELISA and so on.

Specifications

Product Name

Hsp27 Mouse mAb - 一次抗体, specific to Hsp27

同義語

Heat shock 27kDa protein antibody | 28 kDa heat shock protein antibody | CMT2F antibody | DKFZp586P1322 antibody | epididymis secretory protein Li 102 antibody | Estrogen regulated 24 kDa protein antibody | Estrogen-regulated 24 kDa protein antibody | Hea

仕様と純度

Animal Free,Carrier Free,ExactAb™,Azide Free,Validated,High Performance,PBS Only,≥95%(SDS-PAGE),1.0 mg/mL

生化学的・生理学的メカニズム

Small heat shock protein which functions as a molecular chaperone probably maintaining denatured proteins in a folding-competent state. Plays a role in stress resistance and actin organization. Through its molecular chaperone activity may regulate numerou

宿主種

マウス

特異性

Hsp27

免疫原

Recombinant protein corresponding to Human Hsp27

ポジティブコントロール

WB: MCF-7, HeLa, K562, A431, A549 and LNCaP whole cell lysates Flow Cytometry: HeLa cells

活用

Unconjugated

等級

Animal Free, Azide Free, Carrier Free, ExactAb™, High Performance, PBS Only, Validated

ライトチェーン・タイプ

kappa

SDS-PAGE

150.0 KDa

精製方法

Protein A purified

集中

1.0 mg/mL

保管と配送

保管条件

Store at -20°C,Avoid repeated freezing and thawing

入荷

Ice chest + Ice pads

安定性と保管

Store at 4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.

画像

Western Blot: Hsp27 Mouse mAb (Ab210504)

Hsp27 Mouse mAb (Ab210504) - Western Blot
All lanes: Hsp27 Mouse Antibody (Ab210504) at 1/2000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution

Predicted band size: 23 kDa
Observed band size: 27 kDa
Exposure time: 1.7 s 

Western Blot: Hsp27 Mouse mAb (Ab210504)

Hsp27 Mouse mAb (Ab210504) - Western Blot
All lanes: Hsp27 Mouse Antibody (Ab210504) at 1/20000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution

Predicted band size: 23 kDa
Observed band size: 26 kDa
Exposure time: 2.8 s 

Flow Cytometry: Hsp27 Mouse mAb (Ab210504)

Hsp27 Mouse mAb (Ab210504) - Flow Cytometry 
Flow Cytometry analysis of HeLa cells stained with Hsp27 Mouse mAb (Ab210504) (Red) at 0.125 μg/mL for 1 hour at 4°C. Cells were fixed and permeabilized with 100% methanol. The Goat Anti-Mouse IgG H&L (PE) (Ab179006) was used as the secondary antibody. Unlabeled sample (Black) was used as a negative control. The mouse IgG2b was used as an isotype control (Blue).​

適用＃テキヨウ＃

適用＃テキヨウ＃

Dilution info

WB

1/1000 - 1/20000

Flow Cytometry

1/1000 - 1/100,000

関連ターゲット（人間）

HSPB1 Tchem Heat shock protein beta-1 (0 Activities)

Discover Target: HSPB1

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

作用メカニズム

よくある質問

What does Animal Free mean?

Animal Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

What does Carrier Free mean?

Carrier Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (Hsp27 Mouse mAb); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.