Human Elastin (elastin) ELISA Kit

Cat. No.: EJ1514107

GRADE & PURITY BioReagent

概要

This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Human elastin. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Human elastin present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.

Specifications

仕様と純度

BioReagent

等級

BioReagent

アプリケーション

ELISA

サンプルタイプ

プラズマ, 細胞培養上清, 細胞溶解液, 組織ホモジネート, 血清

検出範囲

0.46ng/mL~30ng/mL

検出された生物種

Human

特異性

Detecting Human elastin in samples, with no significant cross-reactivity to other homologs.

Detection instrument

Microplate reader

Detection wavelength

450nm

Repeatability

The intra- and inter-assay coefficients of variation (CV) are both <10%.

Detection duration

3.5 hours, not including equilibration and sample preparation time.

保管と配送

保管条件

Store at 2-8°C

入荷

Wet ice

安定性と保管

Store at 2-8℃ for 6 months.