BioReagent BioReagent ベースライン干渉を最小限に抑える必要がある、感度の高いクロマトグラフィーおよび分析ワークフロー向け。
Store at 2-8°C Wet ice に出荷。正確な仕様については、ロット固有の COA をご確認ください。
SDS、COA、データシート、仕様書をダウンロードできます。ロット固有の COA はロット番号検索で入手可能です。
クロマトグラフィー、有機合成、クロスカップリング反応に関する 0 件の査読済み論文で引用されています。
This kit employs a sandwich enzyme-linked immunosorbent assay (ELISA) for the quantitative detection of Human THBD concentration in samples. The Human THBD capture antibody is pre-coated onto the microtiter plate. Upon addition of samples or standards, Human THBD present therein binds to the immobilized capture antibody, while other unbound components are removed through washing. Subsequently, a biotin-conjugated detection antibody specific for Human THBD is added. This antibody binds to the captured Human THBD, forming a "sandwich" immunocomplex. Any excess, unbound material is again removed by washing. Next, an enzyme conjugate (typically Streptavidin-Horseradish Peroxidase, SA-HRP) is added. The biotin on the detection antibody and streptavidin on the enzyme conjugate exhibit high-affinity binding, thereby linking the HRP enzyme to the immunocomplex. Following another wash step to remove unbound conjugate, a colorimetric substrate (e.g., TMB) is added. If Human THBD is present in the sample, the HRP linked to the complex catalyzes the oxidation of the colorless substrate, yielding a blue product. The reaction is then stopped by adding a stop solution (usually an acid), which changes the color from blue to yellow. The optical density (OD) of each well is measured at 450 nm using a microplate reader. The concentration ofHuman THBD is directly proportional to the OD450 value. A standard curve is generated by assaying known concentrations ofHuman THBD, and the concentration of Human THBD in unknown samples is interpolated from this curve based on their OD values.
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