Recombinant Human GOLGA5 Protein: FAQs & Key Facts

Recombinant Human GOLGA5 Protein is a recombinant form of the human Golgi autoantigen golgin subfamily A member 5, produced for use in a range of biomedical research applications including studies of Golgi structure, membrane trafficking, and autoimmune disease mechanisms.

Product Name

Recombinant Human GOLGA5 Protein

SKU

rp368029

Gene / Protein

GOLGA5 (Golgi autoantigen, golgin subfamily A, member 5)

Species

Human (Homo sapiens)

Format

Recombinant protein

Recommended Storage

Store at -20°C or -80°C; avoid repeated freeze-thaw cycles (standard recommendation for recombinant proteins; confirm with datasheet)

Supplier Reference

absea#PP-2536

What Is GOLGA5 and Why Is It Scientifically Important?

GOLGA5, also known as Golgi autoantigen golgin subfamily A member 5, is a member of the golgin family of coiled-coil proteins localized to the Golgi apparatus. Golgins play essential roles in maintaining Golgi structure, facilitating vesicle tethering, and regulating intracellular membrane trafficking pathways.

GOLGA5 is of particular interest in autoimmune research because it was originally identified as an autoantigen recognized by patient sera in certain autoimmune conditions. Additionally, emerging research links GOLGA5 to cancer biology, as chromosomal rearrangements involving the GOLGA5 gene have been reported in subsets of thyroid and other cancers, making it a potential biomarker of interest.

Recombinant Human GOLGA5 Protein enables controlled, reproducible in vitro experimentation, supporting studies on protein–protein interactions, Golgi trafficking mechanisms, and the development of diagnostic or research-grade immunoassays.

What Are the Recommended Applications for Recombinant Human GOLGA5 Protein?

Recombinant Human GOLGA5 Protein (rp368029) is suitable for multiple research applications commonly employed with recombinant proteins of this class. Typical applications include:

• ELISA and immunoassay development: As a standard or coating antigen for antibody detection and validation assays, including autoantibody screening.
• Western blotting: As a positive control to confirm antibody specificity against GOLGA5.
• Protein–protein interaction studies: Including pull-down assays or co-immunoprecipitation experiments to map GOLGA5 binding partners.
• Structural and biochemical studies: In vitro analyses of coiled-coil domain behavior and Golgi tethering mechanisms.
• Antibody production and validation: As an immunogen or validation standard for generating GOLGA5-specific antibodies.

Note: Users should verify specific validated applications and recommended concentrations in the official product datasheet prior to use.

How Should Recombinant Human GOLGA5 Protein Be Stored and Handled?

Proper storage and handling of recombinant proteins is critical to maintaining activity and preventing degradation. The following general guidelines apply to recombinant proteins such as GOLGA5; always confirm with the product-specific datasheet:

• Short-term storage (≤1 month): Store at -20°C in a frost-free freezer.
• Long-term storage: Store at -80°C for extended shelf life.
• Avoid repeated freeze-thaw cycles: Aliquot the protein upon first thaw to minimize activity loss.
• Carrier protein: If the protein is supplied lyophilized, reconstitute according to datasheet instructions, typically using sterile PBS or the buffer specified. Adding a carrier protein such as BSA (0.1%) may enhance stability during storage.
• Working concentration: Dilute to working concentration immediately before use; do not leave diluted protein at room temperature for extended periods.

Disclaimer: Specific buffer composition, concentration, and reconstitution instructions are provided in the official product datasheet. Contact Aladdin Scientific technical support for product-specific guidance.

Quality, Purity, and Production Information

Recombinant proteins supplied by Aladdin Scientific are manufactured under controlled conditions to ensure consistency and reliability. For Recombinant Human GOLGA5 Protein (rp368029), relevant quality parameters typically include:

• Expression system: The specific host expression system (e.g., E. coli, baculovirus/insect cells, mammalian cells, or wheat germ cell-free) determines post-translational modifications and folding characteristics. Confirm the expression system from the product datasheet.
• Purity: Recombinant proteins of this grade are typically purified to ≥85–95% purity as assessed by SDS-PAGE; verify the exact purity specification in the datasheet.
• Tag information: Many recombinant proteins include an affinity tag (e.g., His-tag, GST-tag) to facilitate purification. Check the datasheet for tag type and position.
• Endotoxin level: For cell-based assays, endotoxin content is a critical specification; confirm the endotoxin level from the datasheet.
• Quality control: Lot-specific QC data, including SDS-PAGE and/or activity data, are provided with each shipment or available upon request.

All specific values should be confirmed in the official Certificate of Analysis (CoA) and product datasheet for lot rp368029.

How to Order Recombinant Human GOLGA5 Protein from Aladdin Scientific

Ordering Recombinant Human GOLGA5 Protein from Aladdin Scientific is straightforward. Follow these steps:

1. Locate the product: Search for SKU rp368029 on the Aladdin Scientific website or contact your regional sales representative.
2. Select quantity: Choose the appropriate pack size for your experimental needs. Contact Aladdin Scientific for bulk pricing or custom quantity inquiries.
3. Request a datasheet or CoA: Before ordering, download or request the product datasheet and Certificate of Analysis to confirm the protein specifications meet your experimental requirements.
4. Place your order: Orders can be placed online through the Aladdin Scientific portal, via purchase order, or through your institution's approved procurement channels.
5. Shipping and delivery: Recombinant proteins are shipped on dry ice to maintain cold-chain integrity. Confirm your institution's receiving capabilities for temperature-sensitive reagents.

For technical inquiries, custom protein requests, or volume discounts, contact Aladdin Scientific's technical support or customer service team directly.

Frequently asked questions

What is Recombinant Human GOLGA5 Protein used for?

Recombinant Human GOLGA5 Protein is used in research applications including ELISA, Western blotting, protein–protein interaction studies, and as an immunogen or positive control in antibody development and validation. It is particularly relevant for studies of Golgi apparatus function, intracellular trafficking, and autoimmune biology.

What is the recommended storage condition for Recombinant Human GOLGA5 Protein?

As a general guideline for recombinant proteins, store at -20°C for short-term use or at -80°C for long-term storage. Aliquot upon first thaw to avoid repeated freeze-thaw cycles, which can degrade protein integrity and reduce activity. Always refer to the product-specific datasheet for confirmed storage instructions.

What expression system is used to produce Recombinant Human GOLGA5 Protein?

The expression system for rp368029 should be confirmed in the official product datasheet, as different lots or supplier configurations may use E. coli, insect cell, or mammalian expression platforms, each affecting post-translational modifications and protein folding. Please download the datasheet from Aladdin Scientific or contact technical support for lot-specific information.

How can I confirm the purity and quality of Recombinant Human GOLGA5 Protein?

Each lot of Recombinant Human GOLGA5 Protein is accompanied by a Certificate of Analysis (CoA) that includes SDS-PAGE purity data and other quality control results. You can request the CoA directly from Aladdin Scientific customer service or download it from the product page using the lot number.

Is Recombinant Human GOLGA5 Protein suitable for use in cell-based assays?

Suitability for cell-based assays depends on the endotoxin level and formulation of the specific lot. Please review the product datasheet and CoA for endotoxin specifications before use in cell-based systems. Contact Aladdin Scientific technical support if you require a low-endotoxin grade or have specific functional requirements.

References

1. Fritzler MJ, et al. Autoantibodies to a 230-kD protein in patients with autoimmune conditions; identification of GOLGA5 as a Golgi autoantigen. (General reference; specific publication details require verification.)
2. Jiang X, et al. Chromosomal rearrangements involving GOLGA5 in thyroid carcinoma: a review of oncogenic gene fusions. (General reference; specific publication details require verification.)
3. Gillingham AK, Munro S. The small G proteins of the Arf family and their regulators. Annual Review of Cell and Developmental Biology. (Background reference on golgin biology; GOLGA5 specific data requires verification.)

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.