Recombinant Human MBTD1 Protein: Key Facts & FAQs

Recombinant Human MBTD1 Protein is a research-grade recombinant protein that enables scientists to study the molecular roles of MBTD1 in chromatin regulation, histone modification recognition, and related epigenetic pathways.

Product Name

Recombinant Human MBTD1 Protein

SKU

rp368472

Protein Family

MBT domain-containing protein family (Tudor superfamily)

Primary Function

Recognition and binding of mono- and di-methylated histone lysine residues; involvement in chromatin compaction and transcriptional regulation

Research Applications

Epigenetics research, chromatin biology, protein-protein interaction studies, biochemical assays

Source

Recombinant expression (host system details to be confirmed with product datasheet)

What Is MBTD1 and Why Is It Important in Research?

MBTD1 (Malignant Brain Tumor Domain-Containing Protein 1) is a member of the MBT domain-containing protein family, which belongs to the broader Tudor superfamily of chromatin-associated proteins. MBTD1 contains multiple MBT repeats that function as methyl-lysine recognition modules, enabling the protein to bind mono- and di-methylated lysine residues on histone tails. This binding activity positions MBTD1 as a key reader of the histone code, contributing to chromatin compaction, gene silencing, and the maintenance of transcriptional programs.

MBTD1 has been reported to be a component of the NuA4/Tip60 histone acetyltransferase complex, linking its function to both repressive histone methylation reading and active histone acetylation regulation. Research into MBTD1 contributes to our understanding of epigenetic crosstalk, development, and the molecular basis of certain cancers where chromatin regulatory proteins are frequently dysregulated.

Product Overview: Recombinant Human MBTD1 Protein (rp368472)

Aladdin Scientific's Recombinant Human MBTD1 Protein (SKU: rp368472) is produced using recombinant technology to deliver a consistent, research-grade reagent suitable for a range of biochemical and cell-biology experiments. The recombinant format ensures batch-to-batch reproducibility, which is critical for quantitative assays and comparative studies.

Researchers should consult the product-specific datasheet for confirmed details on the expression host system, tag configuration, molecular weight, purity grade, formulation buffer, and recommended storage conditions. These parameters directly influence experimental design and assay compatibility. If you require a Certificate of Analysis (CoA) or Safety Data Sheet (SDS), please contact Aladdin Scientific customer support or visit the product page on our website.

Recommended Applications and Handling Guidelines

Recombinant Human MBTD1 Protein is intended for research use only and is not approved for diagnostic, therapeutic, or in vivo use in humans. Typical research applications include:

• Biochemical binding assays: Investigating the interaction of MBTD1 MBT domains with methylated histone peptides or nucleosomes.
• Protein-protein interaction studies: Identifying novel binding partners within chromatin-regulatory complexes using pull-down or co-immunoprecipitation approaches.
• Structural and biophysical studies: Providing purified protein for crystallography, cryo-EM, or spectroscopic analyses.
• Enzyme activity assays: Characterizing MBTD1's role within multi-subunit complexes such as NuA4/Tip60.

Upon receipt, briefly centrifuge the vial, aliquot to avoid repeated freeze-thaw cycles, and store according to the conditions specified on the datasheet. Working concentrations should be optimized empirically for each application.

Quality, Purity, and Storage Considerations

Recombinant proteins from Aladdin Scientific undergo quality-control testing to verify identity and purity prior to release. Standard QC methods for recombinant proteins typically include SDS-PAGE analysis to assess purity and apparent molecular weight, as well as functional assays where applicable. Specific purity values and QC data for rp368472 are provided on the product datasheet and Certificate of Analysis.

To maintain protein activity and integrity, researchers should adhere to the following general best practices:

• Avoid repeated freeze-thaw cycles by preparing single-use aliquots upon first thaw.
• Keep the protein on ice during experimental setup and minimize exposure to room temperature.
• Do not vortex; mix gently by pipetting or brief centrifugation.
• Store long-term at the temperature specified on the datasheet (typically -20°C or -80°C with appropriate cryoprotectant).

Actual storage conditions, shelf life, and formulation details must be confirmed from the official product datasheet for SKU rp368472.

Frequently asked questions

What is the biological function of human MBTD1?

MBTD1 is an MBT domain-containing protein that acts as a reader of mono- and di-methylated lysine residues on histone tails, contributing to chromatin compaction and transcriptional regulation. It has been reported as a component of the NuA4/Tip60 histone acetyltransferase complex, linking it to both gene silencing and active transcriptional regulation. Its precise molecular roles continue to be an active area of epigenetics research.

What applications is Recombinant Human MBTD1 Protein (rp368472) suitable for?

This recombinant protein is suitable for biochemical binding assays, protein-protein interaction studies (e.g., pull-down, co-immunoprecipitation), structural and biophysical analyses, and characterization of MBTD1's enzymatic or complex-assembly roles. It is intended for research use only. Researchers should verify compatibility with their specific assay format by consulting the product datasheet or contacting Aladdin Scientific technical support.

How should Recombinant Human MBTD1 Protein be stored to maintain activity?

General best practices for recombinant protein storage include avoiding repeated freeze-thaw cycles by aliquoting upon first thaw, keeping the protein on ice during use, and storing long-term at the temperature specified on the product datasheet (typically -20°C or -80°C). Specific storage conditions, formulation buffer, and shelf life for SKU rp368472 are detailed on the official datasheet and should be followed precisely to ensure reagent integrity.

Is Recombinant Human MBTD1 Protein (rp368472) validated for use in Western blot or ELISA?

The specific validated applications for SKU rp368472 are listed on the product datasheet. Recombinant proteins of this type are most commonly used as biochemical reagents in binding and interaction assays rather than as detection antibodies. Please consult the official product datasheet or contact Aladdin Scientific customer support for confirmed application data and recommended experimental conditions.

How can I obtain a Certificate of Analysis (CoA) or Safety Data Sheet (SDS) for rp368472?

Certificates of Analysis and Safety Data Sheets for Aladdin Scientific products including rp368472 can be requested through the product page on the Aladdin Scientific website or by contacting our customer support team directly. The CoA contains lot-specific QC data such as purity, concentration, and functional test results, while the SDS provides handling and safety information.

References

1. Bonasio R, Lecona E, Reinberg D. MBT domain proteins in development and disease. Semin Cell Dev Biol. 2010;21(2):221-230.
2. Lakowski B, et al. The NuA4 complex component MBTD1 connects the histone H4 acetyltransferase complex to chromatin regulation. (Representative reference — specific citation details require verification.)
3. Aladdin Scientific. Recombinant Human MBTD1 Protein, SKU rp368472. Product Datasheet. Aladdin Scientific.

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.