KRAS Mouse mAb - 一次抗体, specific to KRAS

Cat. No.: Ab112385

GRADE & PURITY Validated, ExactAb™, See COA

Application

IHC, WB

Host species

マウス

Reactive species

マウス, 人間, 助言

Isotype

Mouse IgG2b

Clone Number

OTI2C1

Conjugation

Unconjugated

概要

Mouse anti Human KRAS Antibody, Monoclonal (OTI2C1), could be used for WB, IHC and so on.

Specifications

Product Name

KRAS Mouse mAb - 一次抗体, specific to KRAS

同義語

c Ki ras2 antibody | c Kirsten ras protein antibody | c-K-ras antibody | c-Ki-ras antibody | Cellular c Ki ras2 proto oncogene antibody | Cellular transforming proto oncogene antibody | CFC2 antibody | cK Ras antibody | GTPase KRas antibody | K RAS p21 pr

仕様と純度

ExactAb™, Validated, See COA

生化学的・生理学的メカニズム

Ras proteins bind GDP/GTP and possess intrinsic GTPase activity.

宿主種

マウス

特異性

KRAS

免疫原

Full length recombinant protein of human KRAS.

ポジティブコントロール

WB: HepG2, HeLa, A549, MDCK, PC12, MCF7, K562, Ramos, 293T, COS7 and Jurkat lysates. IHC: Human normal colon tissue.

活用

Unconjugated

等級

ExactAb™, Validated

SDS-PAGE

150 kDa

精製方法

Protein G purified

集中

See COA

保管と配送

形状

Liquid

保管条件

Store at -20°C

入荷

Ice chest + Ice pads

安定性と保管

Store at 2-8℃ short term (1-2 weeks). Store at -20℃ long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

画像

Western Blot: KRAS Mouse mAb (Ab112385)

KRAS Mouse mAb (Ab112385) - Western Blot
All lanes: KRAS Mouse mAb (Ab112385) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution
Predicted band size: 22 kDa
Observed band size: 21 kDa
Exposure time: 15.0 s

IHC: KRAS Mouse mAb (Ab112385)

KRAS Mouse mAb (Ab112385) - IHC
Immunohistochemistry analysis of paraffin-embedded human normal colon labelling KRAS with KRAS Mouse mAb (Ab112385) at 1/100 dilution. The section was incubated with KRAS Mouse mAb (Ab112385) for 1 h at room temperature. Results show nuclear and cytoplasmic staining. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.​

IHC: KRAS Mouse mAb (Ab112385)

KRAS Mouse mAb (Ab112385) - IHC
Immunohistochemical staining of paraffin-embedded human normal brain labelling KRAS with KRAS Mouse mAb (Ab112385) at 1/100 dilution. The section was incubated with KRAS Mouse mAb (Ab112385) for 1 h at room temperature. Results show nuclear and cytoplasmic staining. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

IHC: KRAS Mouse mAb (Ab112385)

KRAS Mouse mAb (Ab112385) - IHC
Immunohistochemical staining of paraffin-embedded human normal brain labelling KRAS with KRAS Mouse mAb (Ab112385) at 1/100 dilution. The section was incubated with KRAS Mouse mAb (Ab112385) for 1 h at room temperature. Results show nuclear and cytoplasmic staining. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

IHC: KRAS Mouse mAb (Ab112385)

KRAS Mouse mAb (Ab112385) - IHC
Immunohistochemical staining of paraffin-embedded human normal kidney labelling KRAS with KRAS Mouse mAb (Ab112385) at 1/100 dilution. The section was incubated with KRAS Mouse mAb (Ab112385) for 1 h at room temperature. Results show nuclear and cytoplasmic staining. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

適用＃テキヨウ＃

• 適用＃テキヨウ＃: Dilution info
• WB:

  1/2000;

• IHC:

  1/100;

関連ターゲット（人間）

KRAS Tclin GTPase KRas (0 Activities)

Discover Target: KRAS

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

よくある質問

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

How should this product be stored?

Store at ?20 °C. Freezer storage is required to maintain the specified shelf life.

What does Validated mean?

Validated indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (KRAS Mouse mAb); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.