GRADE & PURITYBioReagent?BioReagent grade — tested suitable for life-science and molecular-biology use. Use for cell culture, assays, and biochemical work needing biological compatibility.for Fluorescence analysis?Fluorescence-analysis grade — very low fluorescent impurities for clean spectra. Use in fluorescence assays where background interferes.for Microscopy?Microscopy grade — reagents/stains suited to sample prep and imaging. Use in microscopy where clarity and low background are needed.≥98%(HPLC)
BioReagent, for Fluorescence analysis, for Microscopy, ≥98%(HPLC) BioReagent,蛍光分析用,顕微鏡用 for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at 2-8°C,Protected from light,Desiccated Ships Wet ice Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
概要
L-ANAP (ANAP) is a bright fluorescent amino acid that can be site-specifically incorporated into proteins using amber stop codon suppression.
Application
L-ANAP may be used to fluorescently label proteins and study its localization and dynamics within live mammalian cells.
Biochem/physiol Actions
L-ANAP (ANAP), a fluorescent structural probe, enables real-time monitoring of ion channel dynamics through site-specific incorporation and environmental sensitivity. ANAP features optimal spectral properties with λex ~ 360 nm and environment-dependent λem ~ 490 nm, Φ of 0.22–0.5, and an ϵ of 17.5 mM⁻¹ cm⁻¹ in water. Due to its environmental sensitivity and ability to serve as a FRET donor, ANAP enables simultaneous monitoring of protein conformational changes, ligand binding, protein-protein interactions, and temperature sensitivity while maintaining channel function. ANAP’s versatility is demonstrated through successful applications in various cell types (Xenopus oocytes, HEK-293, CHO, tSA-201 cells), where it can be combined with electrical recordings to provide direct correlation between structural states and channel function. ANAP’s small size, environment-sensitive fluorescence, and the capability to introduce it at specific sites in the proteome through simple mutagenesis make it a unique and valuable tool for eukaryotic cell biology.
Specifications
仕様と純度
BioReagent, for Fluorescence analysis, for Microscopy, ≥98%(HPLC)
安定性と保管
Store at 2-8℃ long term (12 months). Store in the dark. Desiccated.
保管条件
Store at 2-8°C,Protected from light,Desiccated
入荷
Wet ice
この製品は冷冷この冷このこの製品の運送が必要です。地上およびその他の経済サービスは利用できません。
等級
BioReagent, 蛍光分析用, 顕微鏡用
純度
≥98%(HPLC)
Product Line
生物製品
名前と識別子
代替CAS番号
1313516-26-5 (free base)
分子量
386.32
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
Products are supplied to verified businesses, institutions, and qualified professionals for research and development use only. Not for use in humans, animals, diagnosis, or therapy.
