Lichen Red Ethanol Solution (1%) - BioReagent,Suitable for microbiology,Biological Stain,for microscopy,1% , CAS No.1400-62-0

CAS: 1400-62-0 Cat. No.: L1508167 分子式: C₂₈H₂₄N₂O₇ 分子量: 500.5 Beilstein Registry Number: 6885 EC番号: 215-750-6 PubChem CID: 72685

GRADE & PURITY BioReagent, Suitable for microbiology, Biological Stain, for Microscopy, 1%

Synonyms

Orcein Ethanol Solution | Orcein Ethanol Staining Solution

Storage

Store at 2-8°C,Protected from light

Shipped In

Wet ice

Application

Microbial staining

概要

  Orcein, also known as lichenin, has a CAS number of 1400-62-0 and a molecular weight of 500.51. It is a biological mordant that can be used for staining HBsAg, flagella, and other structures.

  Lichen Red Ethanol Solution (1%), also known as Acidified Orcein Staining Solution, is mainly prepared from orcein, hydrochloric acid, ethanol, deionized water and other components. The solution is acidic, featuring simple operation and high specificity, while the staining effect may vary with different batches of raw materials. It can be used for the staining of Hepatitis B Virus Surface Antigen (HBsAg), as well as the staining of lipofuscin and elastic fibers. This reagent is intended for research use only and not for clinical diagnosis or any other purposes.

Operating Procedures (For Reference Only):

1. Fix the tissues in 10% neutral formalin fixative, followed by routine dehydration and embedding.

2. Cut the sections at a thickness of 4 µm, then perform routine dewaxing to water with xylene or environment-friendly dewaxing and clearing solution.

3. Immerse the sections in acidified potassium permanganate solution (self-prepared) for oxidation for 5 minutes, then rinse briefly with water.

4. Transfer the sections into 1% oxalic acid solution (self-prepared) for bleaching for 2 minutes to remove the coloration caused by potassium permanganate, followed by rinsing under running water for 2 minutes.

5. Rinse the sections briefly in 70% ethanol.

6. Place the sections into orcein ethanol solution, cover them, and stain for 5 hours or longer.

7. Rinse the sections twice in 70% ethanol, 10–30 seconds each time, until no staining solution elutes from the sections.

8. Conduct routine dehydration, clear the sections with xylene or environment-friendly dewaxing and clearing solution, and mount the sections with neutral gum.

Precautions:

1. The staining effect may vary depending on different batches of raw materials.

2. For your safety and health, please wear a lab coat and disposable gloves during operation.

3. Please use the reagent as soon as possible after opening to avoid affecting the results of subsequent experiments.

Specifications

同義語

Orcein Ethanol Solution | Orcein Ethanol Staining Solution

仕様と純度

BioReagent,Suitable for microbiology,Biological Stain,for microscopy,1%

安定性と保管

Store at 2-8℃ long term (12 months). Store in the dark.

保管条件

Store at 2-8°C,Protected from light

入荷

Wet ice

この製品は冷冷この冷このこの製品の運送が必要です。地上およびその他の経済サービスは利用できません。

等級

Biological Stain, BioReagent, Suitable for microbiology, 顕微鏡用

Product Line

biological_products

名前と識別子

異性体SMILES

CC1=CC(=CC(=C1NC2=C(C3=C(C=C2O)OC4=CC(=O)C(=NC5=C(C=C(C=C5C)O)O)C(=C43)C)C)O)O

WGKドイツ

3

PubChem CID

72685

分子量

500.5

Beilstein

6885