MAFK Mouse mAb - 一次抗体, specific to MAFK, Mouse IgG1

Cat. No.: Ab113994

GRADE & PURITY Carrier Free, ExactAb™, Validated, 0.5 mg/mL

Application

Flow Cytometry, WB

Host species

マウス

Reactive species

人間

Isotype

Mouse IgG1

Clone Number

D28S786

Conjugation

Unconjugated

概要

Mouse anti Human MAFK Antibody, Monoclonal (D28S786), could be used for WB, Flow and so on.

Specifications

Product Name

MAFK Mouse mAb - 一次抗体, specific to MAFK, Mouse IgG1

同義語

Erythroid transcription factor NF E2 p18 subunit | Erythroid transcription factor NF-E2 p18 subunit | FLJ32205 | MAFK | MAFK_HUMAN | MGC71717 | Nfe2u | P18 | Transcription factor MafK | V maf musculoaponeurotic fibrosarcoma oncogene homolog K (avian) | Er

仕様と純度

ExactAb™, Validated, Carrier Free, 0.5 mg/mL

生化学的・生理学的メカニズム

Since they lack a putative transactivation domain, the small Mafs behave as transcriptional repressors when they dimerize among themselves. However, they seem to serve as transcriptional activators by dimerizing with other (usually larger) basic-zipper pr

宿主種

マウス

特異性

MAFK

免疫原

KLH-conjugated synthetic peptide of human MAFK.

ポジティブコントロール

WB: Jurkat, HeLa and A549 cell lysates. Flow: HeLa cells.

活用

Unconjugated

等級

Carrier Free, ExactAb™, Validated

アイソタイプ

Mouse IgG1

ライトチェーン・タイプ

kappa

SDS-PAGE

150 kDa

精製方法

Protein G purified

集中

0.5 mg/mL

Product Line

biological_products

保管と配送

形状

Liquid

保管条件

Store at -20°C,Avoid repeated freezing and thawing

入荷

Ice chest + Ice pads

安定性と保管

Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

画像

Western Blot: MAFK Mouse mAb (Ab113994)

MAFK Mouse mAb (Ab113994) - Western Blot
All lanes: MAFK Mouse mAb (Ab113994) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution

Predicted band size: 17 kDa
Observed band size: 19 kDa
Exposure time: 8.2 s

Western Blot: MAFK Mouse mAb (Ab113994)

MAFK Mouse mAb (Ab113994) - Western Blot
All lanes: MAFK Mouse mAb (Ab113994) at 1/2000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution

Predicted band size: 17 kDa
Observed band size: 19 kDa
Exposure time: 100.0 s

Flow Cytometry: MAFK Mouse mAb (Ab113994)

MAFK Mouse mAb (Ab113994) - Flow Cytometry
Flow cytometric analysis of HeLa cells using MAFK Mouse mAb (Ab113994). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were incubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by MAFK Mouse mAb (Ab113994) at 37 °C for 60 min. The secondary antibody Goat Anti-Mouse IgG (H&L) - AF488 was incubated at 37 °C for 40 min. Isotype control antibody (blue line) was used under the same condition.

適用＃テキヨウ＃

適用＃テキヨウ＃

Dilution info

WB

1/500-1/2000

Flow Cytometry

1/10-1/50

関連ターゲット（人間）

MAFK Tbio Transcription factor MafK (0 Activities)

Discover Target: MAFK

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

MAFK Tbio Transcription factor MafK (3 Activities)

Discover Target: MAFK

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

作用メカニズム

よくある質問

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Carrier Free mean?

Carrier Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (MAFK Mouse mAb); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.