MCDB is a type of medium designed for low-protein and serum-free culture of specific cells. MCDB 131 is one of the MCDB series media, developed by Knedler and Ham. It was originally used for the culture of human microvascular endothelial cells (HMVEC). In addition to endothelial cells, MCDB 131 medium is also applicable to other cell types, such as hepatocytes, smooth muscle cells, cardiomyocytes and more.
Product Features:
Different from traditional culture media, MCDB 131 medium contains trace elements, putrescine, adenine, thymine, as well as higher concentrations of amino acids and vitamins. It is commonly used in combination with EGF, hydrocortisone, L-glutamine and low-concentration serum. The serum supplementation concentration should be determined according to the specific cell type.
Product Components:
| Form | Liquid | | Concentration | 1× | | Specification | 500 mL | | pH | 7.2-7.4 | | L-Glutamine | None | | NaHCO₃ | 1176 mg/L | | D-Glucose | 1000 mg/L | | Sodium Pyruvate | 1 mM | | HEPES Buffer | None | | Phenol Red Indicator | 12.4 mg/L |
|
Operating Guidelines:
1. Thaw trypsin (0.25% trypsin with 0.02% EDTA) and complete medium to room temperature in advance.
2. Discard the old culture medium and rinse the cell monolayer with DPBS.
3. Add an appropriate volume of trypsin (e.g., 5 mL for a 75 cm² culture flask) to fully cover the cell monolayer. Incubate at 37°C for 1–3 minutes. Observe under an inverted microscope until cells are completely detached from the flask surface; gently tapping the flask helps cell detachment.
4. Add 5–10 mL of complete medium, rinse the inner wall of the flask and mix well. Collect the cell suspension into a 15 mL centrifuge tube.
5. Centrifuge at 1000 rpm for 5–10 minutes, discard the supernatant, and resuspend the cell pellet with 2–5 mL of complete medium.
6. Determine viable cell density and survival rate. Perform cell seeding, incubation and subculture following standard protocols according to cell type and experimental requirements.
Precautions:
1. Transport at room temperature. Upon receipt, store at 2-8°C in the dark and avoid repeated freeze-thaw cycles. The shelf life is 1 year.
2. This product is sterile filtered; gently invert and mix well before use.
3. Our company shall not be liable for any accidents caused by improper use of this product.
ZJ26F0636928