Methemoglobin Reduction Assay Kit (Colorimetric Method)

Cat. No.: M1522005

GRADE & PURITY BioReagent

概要

The examination of red blood cell enzyme defects can be performed through tests such as the methemoglobin reduction test, ascorbic acid test, and Heinz body detection. This kit adopts the Schumm method. Its detection principle is based on the fact that, in the presence of sufficient NADPH, methemoglobin can be reduced to ferrous hemoglobin by methemoglobin reductase. When the glucose-6-phosphate dehydrogenase (G6PD) content in red blood cells is normal, the amount of NADPH generated through the pentose phosphate pathway is sufficient to complete the above reduction reaction. When the G6PD content in red blood cells is insufficient or deficient, the methemoglobin reduction rate slows down, or reduction may even fail, causing methemoglobin to appear brown. This is detected using a spectrophotometer at a wavelength of 635 nm. This kit is intended for research purposes only and is not suitable for clinical diagnosis or other uses.

Specifications

仕様と純度

BioReagent

等級

BioReagent

アプリケーション

Cell Metabolism

検出方法

Colorimetric Method

Detection instrument

Visible Spectrophotometer

Detection wavelength

635nm

保管と配送

保管条件

Store at 2-8°C,Protected from light

入荷

Wet ice

安定性と保管

Store at 2-8℃ for 6 months. Store in the dark.