MMP2 Mouse mAb - 一次抗体, specific to MMP2, Mouse IgG1

Cat. No.: Ab115657

GRADE & PURITY Carrier Free, Validated, ExactAb™, High Performance, See COA

Application

IF/ICC, IHC, WB

Host species

マウス

Reactive species

マウス, 人間, 助言

Isotype

Mouse IgG1

Clone Number

C1

Conjugation

Unconjugated

概要

Mouse anti Human MMP2 Antibody, Monoclonal (C1), could be used for WB, IHC and so on.

Specifications

Product Name

MMP2 Mouse mAb - 一次抗体, specific to MMP2, Mouse IgG1

同義語

72 kDa gelatinase | CLG4 | CLG4A72 kDa type IV collagenase | collagenase type IV-A | EC 3.4.24 | EC 3.4.24.24 | Gelatinase A | matrix metallopeptidase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IVcollagenase) | matrix metalloproteinase 2 (gelatinase A,

仕様と純度

Carrier Free, ExactAb™, Validated, High Performance, See COA

生化学的・生理学的メカニズム

Ubiquitinous metalloproteinase that is involved in diverse functions such as remodeling of the vasculature, angiogenesis, tissue repair, tumor invasion, inflammation, and atherosclerotic plaque rupture. As well as degrading extracellular matrix proteins,

宿主種

マウス

特異性

MMP2

免疫原

Recombinant MMP2 expressed in E.coli (AA 110-660)

ポジティブコントロール

WB: RAW 264.7, C6, HUVEC, A431, SK-N-SH and MCF7 cell lysates. IHC: Human kidney tissue. IF/ICC: HeLa cells.

活用

Unconjugated

等級

Carrier Free, ExactAb™, High Performance, Validated

アイソタイプ

Mouse IgG1

ライトチェーン・タイプ

kappa

SDS-PAGE

150 kDa

精製方法

Protein A + Protein G purified

集中

See COA

保管と配送

形状

Liquid

保管条件

Store at -20°C,Avoid repeated freezing and thawing

入荷

Ice chest + Ice pads

安定性と保管

Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

画像

Western Blot: MMP2 Mouse mAb (Ab115657)

MMP2 Mouse mAb (Ab115657) - Western Blot
All lanes: MMP2 Mouse mAb (Ab115657) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/30000 dilution

Predicted band size: 74 kDa
Observed band size: 74 kDa
Exposure time: 29.4 s

IHC: MMP2 Mouse mAb (Ab115657)

MMP2 Mouse mAb (Ab115657) - IHC
DAB staining on IHC
Samples: Human Kidney Tissue
Primary Ab: 20 µg/mL MMP2 Mouse mAb (Ab115657)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.

IF/ICC: MMP2 Mouse mAb (Ab115657)

MMP2 Mouse mAb (Ab115657) - IF/ICC
IF analysis of MMP2 Mouse mAb (Ab115657) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 5% BSA for 1 hour at room temperature. Cells were stained with MMP2 Mouse mAb (Ab115657) at 1/200 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Mouse IgG H&L (FITC) (Ab179003) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (blue).

適用＃テキヨウ＃

• 適用＃テキヨウ＃: Dilution info
• IHC:

  5-20 µg/mL;

• WB:

  0.01-2 µg/mL ;

• IF/ICC:

  5 µg/mL;

関連ターゲット（人間）

MMP2 Tchem 72 kDa type IV collagenase (0 Activities)

Discover Target: MMP2

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

MMP2 Tchem Matrix metalloproteinase-2 (6627 Activities)

Discover Target: MMP2

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

MMP2 Tchem Collagenase (153 Activities)

Discover Target: MMP2

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

MMP2 Tchem Matrix metalloproteinase 2/9 (2 Activities)

Discover Target: MMP2

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

MMP2 Tchem MMP-2/MMP-13 (113 Activities)

Discover Target: MMP2

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

MMP2 Tchem MMP-2/MMP-9 (120 Activities)

Discover Target: MMP2

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

作用メカニズム

よくある質問

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

What does Carrier Free mean?

Carrier Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (MMP2 Mouse mAb); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.