MS pH Gradient Buffer A - BioReagent

Cat. No.: M1523266

GRADE & PURITY BioReagent

Storage

Store at 2-8°C

Shipped In

Wet ice

概要

pH gradient buffer is an innovative elution buffer (or mobile phase) that changes pH linearly for separation and purification of mAb antibodies, glycoproteins and PTM modified proteins by ion exchange (IEX) chromatography. In pH gradient buffer-based IEX chromatography, the pH of the initial buffer is kept at a constant level to ensure that the charged proteins bind to oppositely charged ions of the stationary phase. Then, the protein is eluted by linearly changing the pH of the buffer so that the protein has a zero net charge and can elute from the column. This buffer is suitable for IEX-MS to characterize intact mAb or subunit. Reagent Composition: Each bottle contains 0.3-1 g Ammonium acetate, and 0.2 g Organic acid. Resuspension: Dissolve reagents in 100 mL deionized, double-distilled water. pH Adjustment: At room temperature, slowly add LC grade ammonia adjust pH value to ensure the Buffer A pH=5.5; the Buffer B=8.5 for ready mobile phase of LC. Elution Buffer (mAb): pH Buffer A, pH 5.5 Elution Buffer B (mAb): pH Buffer B, pH 8.5 Linear pH Range: Linear correlation coefficient > 0.99 at pH 5.5 - 8.5 in columns of IEX chromatography. Reference Procedure for mAb subunit and intact protein: Column: SCX, 10 μm, 2.1 * 250 mm or 2.1 * 50 mm Flow rate: 0.3 mL/min Eluent A: 1X mAb pH buffer A (pH 5.5) Eluent B: 1X mAb pH Buffer B (pH 8.5) Gradient: 0 - 1 min, 0% B; 1 - 31 min, 0 - 100% B; 31 - 34 min, 100% B; 34 - 40 min, 0% B Temperature: 30 °C FLR detection: Ex: 280 nm, Em: 360 nm UV detection: 280 nm Load: 0.2 - 10 μg Inject volume: 4 μL

Specifications

仕様と純度

BioReagent

保管条件

Store at 2-8°C

入荷

Wet ice

この製品は冷冷この冷このこの製品の運送が必要です。地上およびその他の経済サービスは利用できません。

等級

BioReagent

よくある質問

What does BioReagent mean?

BioReagent indicates that the product has been processed and tested for biological and biochemical applications. Specifications may include endotoxin limits, microbial controls, sterility, low DNase/RNase/protease activity, and verified biological activity where relevant.

How should this product be stored?

Store at 2–8 °C. Refrigerated storage is required to maintain the specified shelf life.

How is this product shipped?

This product ships chilled on wet ice. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.