グレード＆純度Carrier Free?Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.1.0 mg/mL
WB: A431, Hep G2, HeLa, HEK293 and MOLT-4 cell lysates. IHC: Human kidney tissue. IP: A431 lysates.
活用
Unconjugated
等級
Carrier Free, ExactAb™, Validated
アイソタイプ
Mouse IgG1
ライトチェーン・タイプ
kappa
SDS-PAGE
150 kDa
精製方法
Protein A purified
集中
1.0 mg/mL
Product Line
生物製品
保管と配送
形状
Liquid
保管条件
Store at -80°C,Avoid repeated freezing and thawing
入荷
Dry ice packs + Cold packs
安定性と保管
Store at 2-8 ℃ (3 months). Store at -20 ℃ (12 months). Store at -80 ℃ (36 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
画像
MTHFD2 Mouse mAb (Ab116276) - Western Blot All lanes: MTHFD2 Mouse mAb (Ab116276) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution Predicted band size: 37 kDa Observed band size: 34 kDa Exposure time: 169.7 s
MTHFD2 Mouse mAb (Ab116276) - IP Immunoprecipitation was performed by incubation of 2.5 µg of MTHFD2 Mouse mAb (Ab116276) with A431 lysate containing 200 µg of total protein. After absorption with Protein G beads, the mixture was run on 6-18% SDS-PAGE and blotted onto nitrocellulose membrane. MTHFD2Mouse mAb (Ab116276) at 1 µg/mL was used as the primary antibody and peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. Lane 1: A431 lysateLane 2: MTHFD2 immunoprecipitated from A431 lysate by MTHFD2 Mouse mAb (Ab116276) Lane 3: IgG isotype control antibody instead of MTHFD2 Mouse mAb (Ab116276) in A431 lysate. Result: MTHFD2 Mouse mAb (Ab116276) can immunoprecipitate MTHFD2.
MTHFD2 Mouse mAb (Ab116276) - IHC IHC-P analysis of kidney tissue by MTHFD2 Mouse mAb (Ab116276).IHC-P was performed using sections of the formalin-fixed paraffin-embedded kidney tissue. Antigen was retrieved through addition of boiling Tris/EDTA buffer pH 9.0 in a pressure cooker for 3 min. Endogenous peroxidase activity was quenched by incubating the sections with 3% H₂O₂ for 30 min at room temperature. The sections were then incubated with MTHFD2 Mouse mAb (Ab116276) at 5 µg/mL at room temperature for 1 h. Poly-peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. Diaminobenzidine was used as the chromogen. The section was counterstained with hematoxylin. A tissue section incubated with phosphate-buffered saline followed by incubation with the secondary antibody was used as the background control.Result: Cells in tubules are positively stained at the cytoplasm.
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よくある質問
What does ExactAb? mean?
ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.
What does Carrier Free mean?
Carrier Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.
How should this product be stored?
Store at ?80 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.
How is this product shipped?
This product ships frozen with dry ice and cold packs. Unpack on arrival and transfer it to the storage condition stated above; handle dry ice with insulated gloves in a ventilated area.
What documentation is provided?
Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.
Which species reactivity has this antibody been validated against?
Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (MTHFD2 Mouse mAb); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.
Products are supplied to verified businesses, institutions, and qualified professionals for research and development use only. Not for use in humans, animals, diagnosis, or therapy.
