Aladdin's EMSA Mutant Probe - ARE is a mutant of the ARE consensus oligonucleotide designed for EMSA studies. It can be used as a negative control of EMSA Probe - ARE in cold mutant probe competition reactions.The nucleotide sequence of EMSA Mutant Probe - ARE is as follows
Precautions：
Avoid heating above 40℃, as double-stranded DNA probe will be denatured at high temperatures into single strands that cannot be used in EMSA.For detailed EMSA procedures, please refer to the manual of EMSA kit from .This product is for R&D only. Not for drug, household, or other uses.For your safety and health, please wear a lab coat and disposable gloves during the operation.Instructions for Use：
1.Set up the cold mutant probe competition reaction by adding the reagents in sequence as follows:Nuclease-free Water 4µlEMSA/Gel-Shift Binding Buffer (5X) 2µlNuclear proteins or purified transcription factors 2µlUnlabeled mutant probe 1µlLabeled probe 1µlTotal volume10µl Note: If a larger volume of unlabeled mutant probe is used, the volume of Nuclease-free Water should be reduced accordingly to keep the total reaction volume at 10µl.その他の情報
| 集中 | 1.75μM |
|---|---|
| 仕様と純度 | 1.75μM |
| 安定性と保管 | Stored at -20 ℃, valid for one year. |
| 保管条件 | Store at -20°C |
| 入荷 | Ice chest + Ice pads |
| Product Description | Aladdin's EMSA Mutant Probe - ARE is a mutant of the ARE consensus oligonucleotide designed for EMSA studies. It can be used as a negative control of EMSA Probe - ARE in cold mutant probe competition reactions.The nucleotide sequence of EMSA Mutant Probe - ARE is as follows Precautions： Avoid heating above 40℃, as double-stranded DNA probe will be denatured at high temperatures into single strands that cannot be used in EMSA.For detailed EMSA procedures, please refer to the manual of EMSA kit from .This product is for R&D only. Not for drug, household, or other uses.For your safety and health, please wear a lab coat and disposable gloves during the operation.Instructions for Use： 1.Set up the cold mutant probe competition reaction by adding the reagents in sequence as follows:Nuclease-free Water 4µlEMSA/Gel-Shift Binding Buffer (5X) 2µlNuclear proteins or purified transcription factors 2µlUnlabeled mutant probe 1µlLabeled probe 1µlTotal volume10µl Note: If a larger volume of unlabeled mutant probe is used, the volume of Nuclease-free Water should be reduced accordingly to keep the total reaction volume at 10µl. |
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