Mutant EMSA Probe STAT5 - 1.75μM

Cat. No.: M745649

GRADE & PURITY 1.75μM

Storage

Store at -20°C

Shipped In

Ice chest + Ice pads

概要

Aladdin's EMSA Mutant Probe – STAT5 is a mutant of the STAT5 consensus oligonucleotide designed for EMSA . It can be used as a negative control for EMSA Probe – STAT5 in cold mutant probe competition reactions.The nucleotide sequence of EMSA Mutant Probe – STAT5 is as follows

Precautions：

Avoid heating above 40℃, as double-stranded DNA probe will be denatured at high temperatures into single strands that cannot be used in EMSA.For detailed EMSA procedures, please refer to the manual of EMSA kits from .This product is for R&D only. Not for drug, household, or other uses.For your safety and health, please wear a lab coat and disposable gloves during the operation.

Instructions for Use：

1.Set up the cold mutant probe competition reaction by adding the reagents in sequence as follows:Nuclease-free Water 4µlEMSA/Gel-Shift Binding Buffer (5X) 2µlNuclear proteins or purified transcription factors 2µlUnlabeled mutant probe 1µlLabeled probe 1µlTotal volume10µl Note: If a larger volume of unlabeled mutant probe is used, the volume of Nuclease-free Water should be reduced accordingly to keep the total reaction volume at 10µl.

Specifications

仕様と純度

1.75μM

安定性と保管

Store at -20℃ for up to 1 year.

保管条件

Store at -20°C

入荷

Ice chest + Ice pads

この製品は冷冷この冷このこの製品の運送が必要です。地上およびその他の経済サービスは利用できません。