Native-PAGE Loading Buffer (5X) - BioReagent, for PAGE, for western blot, 5X

Cat. No.: N752097

グレード＆純度 BioReagent, for PAGE, for Western blot, 5X

Synonyms

Native Gel Sample Loading Buffer (5X)

Storage

Protected from light,Store at -20°C,Avoid repeated freezing and thawing

Shipped In

Ice chest + Ice pads

Application

WB

概要

Native-PAGE Loading Buffer (reduced, 5X) is a 5-fold concentrated SDS-PAGE gel electrophoresis loading buffer with bromophenol blue as the dye. It has the function of precipitating protein samples into gel pores and indicating the movement position of protein samples in gel. The buffer does not contain denaturants such as SDS, but contains reducing agents such as DTT/β - mercaptoethanol. It is suitable for the preparation and electrophoresis of non denatured reduced protein samples.
Precautions：

1. Before adding the sample, thaw it at room temperature or 37-40 ℃ for a few minutes and gently shake well to ensure even mixing of the solution;
2. This product is only used for scientific research and cannot be used for human experiments or treatments;
3. For your own safety, please take protective measures such as wearing lab coats and gloves before using the reagents.
Instructions for Use：

1. Reagent preparation
1.1 Mix the protein tracer loading buffer (non denaturing, 5x) with the protein sample in a ratio of 1:4;
2. Protein sample processing
2.1 Centrifuge the protein sample at less than 3000rpm for 30 seconds;
3. Protein loading and electrophoresis
3.1 After centrifugation, take appropriate amount of supernatant with micro sampler and directly add it into the SDS-PAGE gel sampling hole;
3.2 Conduct routine electrophoresis. Generally, stop electrophoresis when the dye reaches 0.5cm-1cm from the bottom of gel.

仕様

同義語

Native Gel Sample Loading Buffer (5X)

仕様と純度

BioReagent, for PAGE, for western blot, 5X

安定性と保管

Store at -20°C long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle. Store in the dark.

保管条件

Protected from light,Store at -20°C,Avoid repeated freezing and thawing

入荷

Ice chest + Ice pads

この製品は冷冷この冷このこの製品の運送が必要です。地上およびその他の経済サービスは利用できません。

等級

BioReagent, for PAGE, ウエスタンブロット用

Product Line

生物製品

名前と識別子

PH

6.2-7.2

化学的性質と物理的性質

• 感度: Light sensitive