OTS193320 - ≥98% , CAS No.2093401-33-1

CAS: 2093401-33-1 Cat. No.: O646630 分子式: C₂₈H₃₀ClN₅O₄ 分子量: 536.02

GRADE & PURITY ≥98%

Storage

Store at -20°C

Shipped In

Ice chest + Ice pads

概要

OTS193320, a imidazo[1,2-a]pyridine compound, is a SUV39H2 methyltransferase activity inhibitor. OTS193320 decreases global histone H3 lysine 9 tri-methylation levels in breast cancer cells and triggers apoptotic cell death. Combination of OTS193320 with Doxorubicin (DOX; HY-15142A) results in reduction of γ-H2AX levels as well as cancer cell viability compared to a single agent OTS193320 or DOX

In Vitro

OTS193320 (0.125-0.5 μM; 24 hours) has growth inhibitory effect on breast cancer cell lines. OTS193320 exhibits a high inhibitory effect against SUV39H2 enzymatic activity (IC 50 =22.2 nM) and a growth suppressive effect of SUV39H2-positive A549 lung cancer cells (IC 50 =0.38 μM). OTS193320 (0.5 μM; 48 hours) induces apoptosis in breast cancer cells. OTS193320 (0.125-0.5 μM; 24 hours) causes attenuation of H3K9me3 levels in a dose-dependent manner. OTS193320 sensitizes breast cancer cells to doxorubicin via attenuation of γ-H2AX. Combination of OTS193320 and doxorubicin (DOX) significantly attenuates cancer cell viability in vitro, compared to single agent treatment of either drug. MCE has not independently confirmed the accuracy of these methods. They are for reference only. Cell Viability AssayCell Line: MCF-7, SK-BR-3, ZR-75-1, T-47D, MDA-MB-231, BT-20 breast cancer cell lines Concentration: 0-1 μM Incubation Time: 72 hours Result: Had Growth inhibitory effect on MCF-7, SK-BR-3, ZR-75-1, T-47D, MDA-MB-231, and BT-20 breast cancer cell lines with IC 50 values from 0.41 to 0.56 μM, respectively. Apoptosis AnalysisCell Line: MDA-MB-231 and BT-20 cells Concentration: 0.5 μM Incubation Time: 48 hours Result: Showed an increase in the number of cells at early- and late-stage apoptosis. Western Blot AnalysisCell Line: MDA-MB-231 and BT-20 cells Concentration: 0.125, 0.25, 0.5 μM Incubation Time: 24 hours Result: Caused attenuation of H3K9me3 levels in a dose-dependent manner.

Form:Solid

IC50& Target:SUV39H2/KMT1B

Specifications

仕様と純度

≥98%

生化学的・生理学的メカニズム

OTS193320, a imidazo[1,2-a]pyridine compound, is a SUV39H2 methyltransferase activity inhibitor. OTS193320 decreases global histone H3 lysine 9 tri-methylation levels in breast cancer cells and triggers apoptotic cell death. Combination of OTS193320 with

保管条件

Store at -20°C

入荷

Ice chest + Ice pads

この製品は冷冷この冷このこの製品の運送が必要です。地上およびその他の経済サービスは利用できません。

アクションタイプ

INHIBITOR

純度

≥98%

名前と識別子

• 分子量: 536.02

化学的性質と物理的性質

• 溶解度: DMSO : 62.5 mg/mL (116.60 mM; ultrasonic and warming and heat to 60°C)

よくある質問

What is the purity of this product?

This product is supplied at ≥98% purity (chemical assay). Lot-specific values are stated on the Certificate of Analysis.

How should this product be stored?

Store at ?20 °C. Freezer storage is required to maintain the specified shelf life.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What are the CAS number, molecular formula and molecular weight?

The CAS Number is 2093401-33-1, the molecular formula is C28H30ClN5O4, and the molecular weight is 536.02 g/mol.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.