PD1/CD279 Mouse mAb - 一次抗体, specific to PD1/CD279, Mouse IgG1

Cat. No.: Ab120764

GRADE & PURITY Carrier Free, ExactAb™, Azide Free, Validated, PBS Only, ≥95%(SDS-PAGE), See COA

Application

Animal Model, Blocking, Flow Cytometry, Functional Assay, Neutralization

Host species

マウス

Reactive species

人間

Isotype

Mouse IgG1

Clone Number

J116

Conjugation

Unconjugated

概要

Mouse anti Human PD1/CD279 Antibody, Monoclonal (J116), could be used for Flow, in vitro PD-1 neutralization, in vivo PD-1 blockade in humanized mice and so on.
Application:
Flow: Use at an assay dependent concentration.
in vitro PD-1 neutralization: Use at an assay dependent concentration.
in vivo PD-1 blockade in humanized mice: Use at an assay dependent concentration.
Protein Function
Possible cell death inducer, in association with other factors.

Specifications

Product Name

PD1/CD279 Mouse mAb - 一次抗体, specific to PD1/CD279, Mouse IgG1

同義語

hSLE1 | CD279 antigen | CD279 | hPD-1 | PD1 | PD-1 | PD1hPD-l | PDCD1 | programmed cell death 1 | programmed cell death protein 1 | Protein PD-1 | SLEB2 | CD279 antibody | hPD-l | CD279 antigen antibody | hPD 1 antibody | hPD l antibody | hPD-1 antibody |

仕様と純度

Carrier Free,ExactAb™,Azide Free,Validated,PBS Only,≥95%(SDS-PAGE),See COA

宿主種

マウス

特異性

PD1/CD279

ポジティブコントロール

IHC-P: Human tonsil tissue.

活用

Unconjugated

等級

Azide Free, Carrier Free, ExactAb™, PBS Only, Validated

アイソタイプ

Mouse IgG1

ライトチェーン・タイプ

kappa

SDS-PAGE

150 kDa

精製方法

Protein G purified

集中

See COA

Product Line

生物製品

保管と配送

形状

Liquid

保管条件

Store at 2-8°C,Avoid repeated freezing and thawing

入荷

Wet ice

安定性と保管

Shipped at 4°C. Store at 4°C long term (12 months). Do not freeze. Upon receipt, it is recommended to aliquot.

画像

Flow Cytometry: PD1/CD279 Mouse mAb (Ab120764)

PD1/CD279 Mouse mAb (Ab120764) - Flow Cytometry
Flow Cytometry analysis of unstimulated (left) or 500 ng/mL Ionomycin (I139530) and 10 ng/mL PMA (P408905) co-stimulated (right) Jurkat cells labelling PD1/CD279 (red) with PD1/CD279 Mouse mAb (Ab120764) at 0.5 μg/mL for 1 hour at 4°C. Goat Anti-Mouse IgG (PE) (Ab179006) at a dilution of 1/1000 was used as the secondary antibody. Blue - Isotype control, mouse IgG (Ab170221). Black - Unlabelled control, cells without incubation with primary antibody.

適用＃テキヨウ＃

適用＃テキヨウ＃

Dilution info

Flow Cytometry

Use at an assay dependent concentration.

in vitro PD-1 neutralization

Use at an assay dependent concentration.

in vivo PD-1 blockade in humanized mice

Use at an assay dependent concentration.

よくある質問

What is the purity of this product, and how is it determined?

This product is supplied at ≥95% purity, determined by SDS-PAGE. Lot-specific values are stated on the Certificate of Analysis.

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Carrier Free mean?

Carrier Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

How should this product be stored?

Store at 2–8 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships chilled on wet ice. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (PD1/CD279 Mouse mAb); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.