PDK2 Antibody - 一次抗体, specific to PDK2, Rabbit IgG

Cat. No.: Ab120993

GRADE & PURITY Validated, 1.0 mg/mL

Application

IHC, WB

Host species

ウサギ

Reactive species

マウス, 人間, 助言

Isotype

Rabbit IgG

Conjugation

Unconjugated

概要

Rabbit anti Human PDK2 Antibody, Polyclonal, could be used for WB, IHC and so on.
Application
WB: 1/500-1/2000
IHC: 1/100 - 1/300
Protein Function
Inhibits the mitochondrial pyruvate dehydrogenase complex by phosphorylation of the E1 alpha subunit, thus contributing to the regulation of glucose metabolism.

Specifications

Product Name

PDK2 Antibody - 一次抗体, specific to PDK2, Rabbit IgG

同義語

PDHK2 | Pyruvate dehydrogenase kinase isoform 2 | pyruvate dehydrogenase kinase, isozyme 2 | Pyruvate dehydrogenase lipoamide kinase isozyme 2, mitochondrial | PDK2_HUMAN | [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 2 antibody | mitochondrial ant

仕様と純度

Validated, 1.0 mg/mL

宿主種

ウサギ

特異性

PDK2

免疫原

A synthetic peptide derived from human PDK2 (AA 260-309)

ポジティブコントロール

WB: Mouse brain, Rat heart lysates. IHC: Human liver cancer tissue.

活用

Unconjugated

等級

Validated

アイソタイプ

Rabbit IgG

SDS-PAGE

150 kDa

精製方法

Immunogen affinity purified

集中

1.0 mg/mL

Product Line

biological_products

保管と配送

形状

Liquid

保管条件

Store at -20°C,Avoid repeated freezing and thawing

入荷

Ice chest + Ice pads

安定性と保管

Store at 4°C short term (1-2 weeks). Store at -20℃ long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

画像

Western Blot: PDK2 Antibody (Ab120993)

PDK2 Antibody (Ab120993) - Western Blot
All lanes: PDK2 Antibody (Ab120993) at 1/500 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/10000 dilution

Predicted band size: 46 kDa
Observed band size: 46 kDa
Exposure time: 100.0 s

IHC: PDK2 Antibody (Ab120993)

PDK2 Antibody (Ab120993) - IHC
Immunohistochemical analysis of paraffin-embedded human liver cancer. PDK2 Antibody (Ab120993) was diluted at 1/200 (4° overnight). Tris-EDTA, pH 9.0 was used for antigen retrieval. Secondary antibody was diluted at 1/200 (room temperature, 45 min). 

適用＃テキヨウ＃

適用＃テキヨウ＃

Dilution info

WB

1/500-1/2000

IHC

1/100-1/300

関連ターゲット（人間）

PDK2 Tchem [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 2, mitochondrial (0 Activities)

Discover Target: PDK2

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

PDK2 Tchem Pyruvate dehydrogenase kinase isoform 2 (894 Activities)

Discover Target: PDK2

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

作用メカニズム

よくある質問

What does Validated mean?

Validated indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (PDK2 Antibody); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.