Peripherin Antibody - 一次抗体, specific to PRPH, Rabbit IgG

Cat. No.: Ab121296

グレード＆純度 Validated, 1.0 mg/mL

Application

IHC, WB

Host species

ウサギ

Reactive species

マウス, 人間, 助言

Isotype

Rabbit IgG

Conjugation

Unconjugated

概要

Rabbit anti Human Peripherin Antibody, Polyclonal, could be used for WB, IHC and so on.
Application
· WB: 1/500-1/2000
· IHC: 1/100-1/300
Protein Function
Class-III neuronal intermediate filament protein.

仕様

Product Name

Peripherin Antibody - 一次抗体, specific to PRPH, Rabbit IgG

同義語

PRPH1 | NEF 4 antibody | NEF4 antibody | Neurofilament 4 (57kD) antibody | Neurofilament 4 antibody | Perf antibody | PERI_HUMAN antibody | Peripherin antibody | PRPH 1 antibody | prph antibody | PRPH1 antibody | NEF4

仕様と純度

Validated, 1.0 mg/mL

宿主種

ウサギ

特異性

PRPH

免疫原

A synthetic peptide derived from human Peripherin (AA 421-470).

ポジティブコントロール

WB: Mouse brain, NIH/3T3 and C6 lysates. IHC: Human brain tissue.

活用

Unconjugated

等級

Validated

アイソタイプ

Rabbit IgG

ライトチェーン・タイプ

kappa

SDS-PAGE

150 kDa

精製方法

Immunogen affinity purified

集中

1.0 mg/mL

Product Line

生物製品

保管と配送

形状

Liquid

保管条件

Store at -20°C,Avoid repeated freezing and thawing

入荷

Ice chest + Ice pads

安定性と保管

Store at -20℃ long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

画像

Western Blot: Peripherin Antibody (Ab121296)

Peripherin Antibody (Ab121296) - Western Blot
All lanes: Peripherin Antibody (Ab121296) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/10000 dilution
Predicted band size: 54 kDa
Observed band size: 57 kDa
Exposure time: 16.2 s

Western Blot: Peripherin Antibody (Ab121296)

Peripherin Antibody (Ab121296) - Western Blot
All lanes: Peripherin Antibody (Ab121296) at 1/1000 dilution
Samples: Lysates at 10 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/10000 dilution
Predicted band size: 54 kDa
Observed band size: 48, 57 kDa. The 57 kDa and 48 kDa isoforms of peripherin observed are consistent with what has been described in the literature (PMID: 20587592, 18287500, 17475883).
Exposure time: 16.2 s

IHC: Peripherin Antibody (Ab121296)

Peripherin Antibody (Ab121296) - IHC
Immunohistochemistry analysis of paraffin-embedded human brain tissue, using Peripherin Antibody (Ab121296). The picture on the right is blocked with the synthesized peptide.

適用＃テキヨウ＃

適用＃テキヨウ＃

Dilution info

WB

1/500-1/2000

IHC

1/100-1/300

関連ターゲット（人間）

PRPH Tbio Peripherin (0 Activities)

Discover Target: PRPH

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

よくある質問

What does Validated mean?

Validated indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (Peripherin Antibody); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.