Phosphatase, Alkaline, CAS No.9001-78-9

CAS: 9001-78-9 Cat. No.: P128629 EC番号: 232-631-4 PubChem CID: 18985873

グレード＆純度 Native, EnzymoPure™, ≥30 units/mg protein (25°C, pH 8.0),from Escherichia coli

Accession #

P00634

Bioactivity

≥30 units/mg protein (25°C, pH 8.0)

概要

Alkaline phosphatase is a broad term associated with non-specific phosphomonoesterases with an alkaline pH optimum.
Alkaline phosphatase is used for conjugation to antibodies and other proteins for ELISA, Western blotting, and histochemical detection. It may be used for protein labeling when high sensitivity is required.Inibibitors: The enzyme is inhibited by chelating agents and inorganic phosphates.

Unit Definitons

E. coli. One Unit hydrolyzes 1µmole of p-nitrophenol phosphate per minute at 25°C, pH 8.0.

仕様

Product Name

Phosphatase, Alkaline, CAS No.9001-78-9

同義語

Alkaline phosphatase | Phosphatase, alkaline | ENTEROSTATIN,HUMAN | alkaline phosphomonoesterase | glycerophosphatase | phosphomonoesterase | Orthophosphoric-monoester phosphohydrolase (alkaline optimum) | alkaline phosphatase | Apase, Escherichia coli |

等級

Native, EnzymoPure™

仕様と純度

EnzymoPure™, Nature, ≥30 units/mg protein (25°C, pH 8.0),from Escherichia coli

生化学的・生理学的メカニズム

Alkaline phosphatase, from Escherichia coli, is a dimeric, non-glycosylated protein which mainly reside in the periplasmic space. Three known isoforms exist. The enzyme requires zinc, and is activated by magnesium. E. coli akaline phosphatase has a broad

生物活性

≥30 units/mg protein (25°C, pH 8.0)

アクセス番号

P00634

CAS

9001-78-9

酵素委員会番号

I.U.B.: 3.1.3.1

分子タイプ

酵素

保管と配送

集中

≥30 units/mg protein (25°C, pH 8.0),from Escherichia coli

保管条件

Store at 2-8°C

入荷

Wet ice

ユニットの定義

One Unit hydrolyzes 1µmole of p-nitrophenol phosphate per minute at 25°C, pH 8.0.